Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 Aug;104(2):217-24. doi: 10.1007/s10482-013-9940-2. Epub 2013 Jun 1.
A Gram-negative, non-motile and rod-shaped bacterial strain, designated BB-MW15(T), was isolated from a tidal flat of the southern coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic taxonomic approach. Strain BB-MW15(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2-3 % (w/v) NaCl. Strain BB-MW15(T) exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Marivita byunsanensis (96.8 %), Thalassobius maritimus (96.6 %), Sulfitobacter marinus (96.4 %) and Marinovum algicola (96.3 %). Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-MW15(T) forms an independent lineage within the evolutionary radiation encompassed by the Roseobacter clade of the class Alphaproteobacteria. Strain BB-MW15(T) contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids detected in strain BB-MW15(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain BB-MW15(T) is 62.7 mol%. Based on phylogenetic, chemotaxonomic and other phenotypic characteristics, strain BB-MW15(T) represents a new genus and a new species within Alphaproteobacteria, for which the name Aestuariihabitans beolgyonensis gen. nov., sp. nov. is proposed. The type strain of A. beolgyonensis is BB-MW15(T) (= KCTC 32324(T) = CCUG 63829(T)).
一株革兰氏阴性、非运动和杆状的细菌菌株，被命名为 BB-MW15(T)，从朝鲜半岛南部海岸的潮滩中分离出来，并通过多相分类方法研究了其分类地位。菌株 BB-MW15(T)在 30°C、pH 值 7.0-8.0 和 2-3%(w/v)NaCl 的存在下生长最佳。菌株 BB-MW15(T)与 Byunsanensis marivita、Thalassobius maritimus、Sulfitobacter marinus 和 Marinovum algicola 的模式菌株的 16S rRNA 基因序列相似度最高，分别为 96.8%、96.6%、96.4%和 96.3%。基于 16S rRNA 基因序列的邻接法、最大似然法和最大简约法系统发育树表明，菌株 BB-MW15(T)在 Alpha 变形菌纲的 Roseobacter 进化辐射范围内形成一个独立的谱系。菌株 BB-MW15(T)含有 Q-10 作为主要的泛醌和 C18:1 ω7c 作为主要的脂肪酸。在菌株 BB-MW15(T)中检测到的主要极性脂是磷脂酰胆碱、磷脂酰甘油、一种未鉴定的胺脂和一种未鉴定的脂。菌株 BB-MW15(T)的 DNA G+C 含量为 62.7mol%。基于系统发育、化学分类和其他表型特征，菌株 BB-MW15(T)代表了 Alpha 变形菌中的一个新属和一个新种，建议将其命名为 Aestuariihabitans beolgyonensis gen. nov.，sp. nov.。A. beolgyonensis 的模式菌株为 BB-MW15(T)=KCTC 32324(T)=CCUG 63829(T)。
