Losi Paola, Al Kayal Tamer, Buscemi Marianna, Foffa Ilenia, Cavallo Aida, Soldani Giorgio
Institute of Clinical Physiology, National Research Council, 54100 Massa, Italy.
Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127 Pisa, Italy.
Nanomaterials (Basel). 2020 Oct 27;10(11):2128. doi: 10.3390/nano10112128.
The present study examined the effects of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate by a combination of electrospinning and spray, phase-inversion method for wound healing. In particular, the poly(ether)urethane layer was obtained using by a spray phase-inversion method and the fibrin fibers network were loaded with platelet lysate by electrospinning. The kinetics release and the bioactivity of growth factors released from platelet lysate-scaffold were investigated by ELISA and cell proliferation test using mouse fibroblasts, respectively. The in-vitro experiments demonstrated that a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate provides a sustained release of bioactive platelet-derived growth factors. The effect of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate on wound healing in diabetic mouse (db/db) was also investigated. The application of the scaffold on full-thickness skin wounds significantly accelerated wound closure at day 14 post-surgery when compared to scaffold without platelet lysates or commercially available polyurethane film, and at the same level of growth factor-loaded scaffold. Histological analysis demonstrated an increased re-epithelialization and collagen deposition in platelet lysate and growth factor loaded scaffolds. The ability of bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate to promote in-vivo wound healing suggests its usefulness in clinical treatment of diabetic ulcers.
本研究通过静电纺丝和喷雾相结合的方法，采用相转化法制备了负载血小板裂解物的双层纤维蛋白/聚（醚）聚氨酯支架，用于伤口愈合。具体而言，聚（醚）聚氨酯层通过喷雾相转化法获得，纤维蛋白纤维网络通过静电纺丝负载血小板裂解物。分别采用酶联免疫吸附测定法（ELISA）和小鼠成纤维细胞增殖试验研究了血小板裂解物-支架释放的生长因子的动力学释放和生物活性。体外实验表明，负载血小板裂解物的双层纤维蛋白/聚（醚）聚氨酯支架能持续释放具有生物活性的血小板衍生生长因子。还研究了负载血小板裂解物的双层纤维蛋白/聚（醚）聚氨酯支架对糖尿病小鼠（db/db）伤口愈合的影响。与未负载血小板裂解物的支架或市售聚氨酯薄膜相比，在手术后第14天，将该支架应用于全层皮肤伤口可显著加速伤口闭合，且与负载生长因子的支架效果相当。组织学分析表明，负载血小板裂解物和生长因子的支架中上皮再形成和胶原沉积增加。负载血小板裂解物的双层纤维蛋白/聚（醚）聚氨酯支架促进体内伤口愈合的能力表明其在糖尿病溃疡临床治疗中的实用性。
