Komaki Hisayuki, Tamura Tomohiko
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, Japan.
Life (Basel). 2020 Oct 27;10(11):257. doi: 10.3390/life10110257.
(1) Background: is a recently established genus belonging to rare actinomycetes. It has been unclear if its members have the capacity to synthesize diverse secondary metabolites. Polyketide and nonribosomal peptide compounds are major secondary metabolites in actinomycetes and expected as a potential source for novel pharmaceuticals. (2) Methods: Whole genomes of NBRC 107702, NBRC 108638, NBRC 108639, and NBRC 105367 were sequenced by PacBio. Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters were bioinformatically analyzed in the genome sequences. (3) Results: These four strains harbored 10, 14, 18 and 14 PKS and NRPS gene clusters, respectively. Most of the gene clusters were annotated to synthesis unknown chemistries. (4) Conclusions: Members of the genus are a possible source for novel and diverse polyketides and nonribosomal peptides.
(1) 背景：[属名]是最近建立的一个属于稀有放线菌的属。其成员是否有能力合成多种次生代谢产物尚不清楚。聚酮化合物和非核糖体肽化合物是放线菌中的主要次生代谢产物，有望成为新型药物的潜在来源。(2) 方法：使用PacBio对[菌株名]NBRC 107702、[菌株名]NBRC 108638、[菌株名]NBRC 108639和[菌株名]NBRC 105367的全基因组进行测序。在基因组序列中对聚酮合酶（PKS）和非核糖体肽合成酶（NRPS）基因簇进行生物信息学分析。(3) 结果：这四株菌株分别含有10个、14个、18个和14个PKS和NRPS基因簇。大多数基因簇被注释为合成未知化学物质。(4) 结论：[属名]属的成员是新型和多样聚酮化合物和非核糖体肽的可能来源。
