Komaki Hisayuki, Ichikawa Natsuko, Hosoyama Akira, Hamada Moriyuki, Harunari Enjuro, Ishikawa Arisa, Igarashi Yasuhiro
Biological Resource Center, National Institute of Technology and Evaluation, Chiba, Japan.
NBRC, Tokyo, Japan.
Stand Genomic Sci. 2016 Oct 22;11:84. doi: 10.1186/s40793-016-0206-2. eCollection 2016.
Here, we report the draft genome sequence of sp. DSW705 (=NBRC 110037), a producer of antitumor cyclic depsipeptides rakicidins A and B, together with the features of this strain and generation, annotation, and analysis of the genome sequence. The 6.8 Mb genome of sp. DSW705 encodes 6,219 putative ORFs, of which 4,846 are assigned with COG categories. The genome harbors at least three type I polyketide synthase (PKS) gene clusters, one nonribosomal peptide synthetase (NRPS) gene clusters, and three hybrid PKS/NRPS gene clusters. A hybrid PKS/NRPS gene cluster encoded in scaffold 2 is responsible for rakicidin synthesis. DNA database search indicated that the biosynthetic gene clusters for depsipeptides bearing 4-amino-2,4-pentadienoate are widely present in taxonomically diverse actinomycetes.
在此，我们报告了抗肿瘤环缩肽拉基杀菌素A和B的产生菌——[具体菌种名称] sp. DSW705（=NBRC 110037）的基因组序列草图，以及该菌株的特征、基因组序列的生成、注释和分析。[具体菌种名称] sp. DSW705的6.8 Mb基因组编码6219个推定的开放阅读框（ORF），其中4846个被归类到COG类别。该基因组含有至少三个I型聚酮合酶（PKS）基因簇、一个非核糖体肽合成酶（NRPS）基因簇和三个杂交PKS/NRPS基因簇。支架2中编码的一个杂交PKS/NRPS基因簇负责拉基杀菌素的合成。DNA数据库搜索表明，带有4-氨基-2,4-戊二烯酸的缩肽生物合成基因簇广泛存在于分类学上不同的放线菌中。
