Komaki Hisayuki, Hosoyama Akira, Igarashi Yasuhiro, Tamura Tomohiko
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.
Microorganisms. 2020 Mar 6;8(3):370. doi: 10.3390/microorganisms8030370.
(1) Background: sp. TP-A0598 derived from seawater produces lydicamycin and its congeners. We aimed to investigate its taxonomic status; (2) Methods: A polyphasic approach and whole genome analysis are employed; (3) Results: Strain TP-A0598 contained ll-diaminopimelic acid, glutamic acid, glycine, and alanine in its peptidoglycan. The predominant menaquinones were MK-9(H) and MK-9(H), and the major fatty acids were C, -C, -C, and -C. sp. TP-A0598 showed a 16S rDNA sequence similarity value of 99.93% (1 nucleottide difference) to NRRL B-2347. The digital DNA-DNA hybridisation value between sp. TP-A0598 and its closely related type strains was 25%-46%. Differences in phenotypic characteristics between sp. TP-A0598 and its phylogenetically closest relative, NBRC 3934, suggested strain TP-A0598 to be a novel species. sp. TP-A0598 and NBRC 3934 harboured nine and 13 biosynthetic gene clusters for polyketides and nonribosomal peptides, respectively, among which only five clusters were shared between them, whereas the others are specific for each strain; and (4) Conclusions: For strain TP-A0598, the name sp. nov. is proposed; the type strain is TP-A0598 (=NBRC 110027).
(1) 背景：源自海水的TP-A0598菌株可产生利迪卡霉素及其同系物。我们旨在研究其分类地位；(2) 方法：采用多相分类法和全基因组分析；(3) 结果：菌株TP-A0598的肽聚糖中含有ll-二氨基庚二酸、谷氨酸、甘氨酸和丙氨酸。主要的甲基萘醌为MK-9(H)和MK-9(H)，主要脂肪酸为C、-C、-C和-C。TP-A0598菌株与NRRL B-2347的16S rDNA序列相似性值为99.93%（相差1个核苷酸）。TP-A0598菌株与其密切相关的模式菌株之间的数字DNA-DNA杂交值为25%-46%。TP-A0598菌株与其系统发育关系最密切的相对菌株NBRC 3934在表型特征上的差异表明菌株TP-A0598是一个新物种。TP-A0598菌株和NBRC 3934菌株分别含有9个和13个聚酮化合物和非核糖体肽的生物合成基因簇，其中只有5个簇是它们共有的，而其他簇则是每个菌株特有的；(4) 结论：对于菌株TP-A0598，提议命名为新物种；模式菌株为TP-A0598 (=NBRC 110027)。
