Emmerich R V, Hirshfield I N
Department of Biological Sciences, St. John's University, Jamaica, New York 11439.
J Bacteriol. 1987 Nov;169(11):5311-3. doi: 10.1128/jb.169.11.5311-5313.1987.
The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.
通过接合和转导相结合的方法，并经二维凝胶电泳进行物理验证，将组成型赖氨酰 - tRNA合成酶基因（lysS）定位在大肠杆菌染色体的62.1分钟处。回复突变分析表明，突变型LysS蛋白的等电点改变和含量较低可能是由于单个突变事件所致。
