Zhao Xiuzhen, Jin Tao, Zheng Chao, Ma Di, Zhang Ying
Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, China.
Department of Neurology, Jining No. 1 People's Hospital, Shandong, China.
Front Neurol. 2020 Oct 2;11:572205. doi: 10.3389/fneur.2020.572205. eCollection 2020.
Follicular helper T (Tfh) cells and follicular regulatory T (Tfr) cells are essential for B cell differentiation, germinal center formation, and humoral immune responses. Immunity and inflammation have been thought to be involved in Parkinson's disease (PD). In this study, we aimed to identify whether circulating Tfh and Tfr (cTfh and cTfr) cells contribute to PD. Thirty-nine PD patients and 26 health controls (HCs) were enrolled. The numbers of cTfh (CD4CXCR5PD-1) cells and cTfr (CD4CXCR5CD25CD127) cells were analyzed via flow cytometry. The serum concentrations of interleukin (IL)-4, IL-10, IL-21, and transforming growth factor (TGF)-β were examined by cytometric bead array. The percentage of cTfh cells among CD4 T cells in PD patients was significantly higher than that in HCs [3.68% (2.64-5.70%) vs. 1.94% (1.32%-2.99%), < 0.001], while the percentage of cTfr cells among CD4 T cells in PD patients was slight decreased but without significance [1.05% (0.62-1.54%) vs. 1.3% (0.63-1.90%), > 0.05]. The percentage of CD19 B cells in peripheral blood mononuclear cells was significantly lower in PD patients than in HCs [5.35% (4.13-9.38%) vs. 8.68% (5.61-12.93%), = 0.014]. The serum concentrations of IL-4, IL-10, IL-21, and TGF-β in PD patients did not differ significantly from those in HCs ( > 0.05). There was a positive trend of the correlation between the number of cTfh and the serum IL-4 concentrations in PD patients ( = 0.032, = 0.353). There was a positive trend of the correlation between the number of cTfr and the serum IL-10 concentrations in PD patients ( = 0.047, = 0.328), A positive trend of the correlation were found for the serum concentration of IL-21 with H-Y stage ( = 0.356, = 0.026) and UPDRS-III score ( = 0.347, = 0.030). These results indicate that an imbalance of cTfh and cTfr cells may be involved in the chronic progression of PD, and IL-21 may be a biomarker for monitoring the severity of this disease.
滤泡辅助性T（Tfh）细胞和滤泡调节性T（Tfr）细胞对于B细胞分化、生发中心形成及体液免疫反应至关重要。免疫和炎症被认为与帕金森病（PD）有关。在本研究中，我们旨在确定循环Tfh和Tfr（cTfh和cTfr）细胞是否与PD有关。招募了39例PD患者和26名健康对照（HC）。通过流式细胞术分析cTfh（CD4CXCR5PD-1）细胞和cTfr（CD4CXCR5CD25CD127）细胞的数量。通过细胞计数珠阵列检测白细胞介素（IL）-4、IL-10、IL-21和转化生长因子（TGF）-β的血清浓度。PD患者CD4 T细胞中cTfh细胞的百分比显著高于HC [3.68%（2.64 - 5.70%）对1.94%（1.32% - 2.99%），<0.001]，而PD患者CD4 T细胞中cTfr细胞的百分比略有下降但无统计学意义[1.05%（0.62 - 1.54%）对1.3%（0.63 - 1.90%），>0.05]。PD患者外周血单个核细胞中CD19 B细胞的百分比显著低于HC [5.35%（4.13 - 9.38%）对8.68%（5.61 - 12.93%），=0.014]。PD患者血清中IL-4、IL-10、IL-21和TGF-β的浓度与HC相比无显著差异（>0.05）。PD患者中cTfh数量与血清IL-4浓度之间存在正相关趋势（=0.032，=0.353）。PD患者中cTfr数量与血清IL-10浓度之间存在正相关趋势（=0.047，=0.328），血清IL-21浓度与H-Y分期（=0.356，=0.026）和统一帕金森病评定量表第三部分（UPDRS-III）评分（=0.347，=0.030）之间存在正相关趋势。这些结果表明，cTfh和cTfr细胞的失衡可能参与了PD的慢性进展，并且IL-21可能是监测该疾病严重程度的生物标志物。
