大多数或所有转运RNA的受体茎中存在一个强溴化乙锭结合位点。
A strong ethidium binding site in the acceptor stem of most or all transfer RNAs.
作者信息
Wells B D, Cantor C R
出版信息
Nucleic Acids Res. 1977;4(5):1667-80. doi: 10.1093/nar/4.5.1667.
Abstract
E. coli unfractionated tRNA and tRNA phe both contain a single strong ethidium binding site. Singlet-singlet energy transfer has been used to measure the distance between this site and dansyl hydrazine covalently attached to the 3' end of the tRNAs. The distance obtained is between 33 and 40 A for both samples. This is completely consistent with results from earlier NMR studies which placed the single, strong ethidium binding site of yeast tRNAphe between base pairs 6 and 7 on the aminoacyl stem. From the known tertiary structure of tRNAphe it is possible to rationalize the unusual affinity of this site and its likely existence in all tRNAs.
摘要
大肠杆菌未分级的转运RNA（tRNA）和苯丙氨酸tRNA都含有一个单一的强溴化乙锭结合位点。单重态-单重态能量转移已被用于测量该位点与共价连接到tRNA 3'端的丹磺酰肼之间的距离。两个样品得到的距离在33至40埃之间。这与早期核磁共振研究的结果完全一致，早期研究将酵母苯丙氨酸tRNA的单一强溴化乙锭结合位点定位在氨酰基茎的碱基对6和7之间。从已知的苯丙氨酸tRNA三级结构可以合理地解释该位点的异常亲和力及其可能在所有tRNA中的存在。
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