ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Lucknow 226 002, Uttar Pradesh, India.
School of Ocean and Earth Science, University of Southampton, Waterfront Campus, European Way, Southampton, SO14 3ZH, United Kingdom; Present address: Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
Genomics. 2021 Jan;113(1 Pt 2):944-956. doi: 10.1016/j.ygeno.2020.10.028. Epub 2020 Oct 28.
Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete.
感染 Aphanomyces invadans 是一种严重的鱼类疾病，具有重大的全球影响。尽管它影响了超过 160 种鱼类，但有些鱼类，如鲤鱼，对 A. invadans 感染具有抗性。在本研究中，我们研究了实验感染 A. invadans 的鲤鱼头肾的转录组。在时间过程分析中，发现了 5288 个差异表达基因（DEGs），其中 731 个基因参与了 21 个免疫途径。对免疫相关 DEGs 的分析表明，有效处理和呈现 A. invadans 抗原、增强吞噬作用、识别病原体相关分子模式以及增加白细胞募集到感染部位有助于鲤鱼抵抗 A. invadans。在此，我们从分子水平上为鲤鱼的抗病机制提供了系统的理解，这为开发这种破坏性鱼类病原菌卵菌的疾病管理策略提供了有价值的资源。
