Akram Saba, Arif Mian Abdur Rehman, Hameed Amjad
Nuclear Institute for Agriculture and Biology College. Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Jhang Road, Faisalabad, Pakistan.
J Appl Genet. 2021 Feb;62(1):27-41. doi: 10.1007/s13353-020-00593-1. Epub 2020 Oct 30.
Wheat is a foremost food grain of Pakistan and occupies a vital position in agricultural policies of the country. Wheat demand will be increased by 60% by 2050 which is a serious concern to meet this demand. Conventional breeding approaches are not enough to meet the demand of growing human population. It is paramount to integrate underutilized genetic diversity into wheat gene pool through efficient and accurate breeding tools and technology. In this study, we present the genetic analysis of a 312 diverse pre-breeding lines using DArT-seq SNPs seeking to elucidate the genetic components of emergence percentage, heading time, plant height, lodging, thousand kernel weight, and yield (Yd) which resulted in detection of 201 significant (p value < 10) and 61 highly significant associations (p value < 1.45 × 10). More importantly, chromosomes 1B and 2A carried loci linked to Yd in two different seasons, and an increase of up to 8.20% is possible in Yd by positive allele mining. We identified seven lines with > 4 positive alleles for Yd whose pedigree carried Aegilops squarrosa as one of the parents providing evidence that Aegilops species, apart from imparting resistance against biotic stresses, may also provide alleles for yield enhancement.
小麦是巴基斯坦最重要的粮食作物，在该国农业政策中占据重要地位。到2050年，小麦需求将增长60%，满足这一需求是一个严峻的问题。传统育种方法不足以满足不断增长的人口需求。通过高效准确的育种工具和技术，将未充分利用的遗传多样性整合到小麦基因库中至关重要。在本研究中，我们利用DArT-seq SNPs对312个不同的预育种系进行了遗传分析，旨在阐明出苗率、抽穗期、株高、倒伏、千粒重和产量（Yd）的遗传组成部分，结果检测到201个显著（p值<10）和61个高度显著的关联（p值<1.45×10）。更重要的是，1B和2A染色体在两个不同季节携带与Yd相关的位点，通过正向等位基因挖掘，Yd可能提高高达8.20%。我们鉴定出7个Yd有>4个正向等位基因的品系，其谱系中携带粗山羊草作为亲本之一，这证明粗山羊草属物种除了赋予对生物胁迫的抗性外，还可能提供提高产量的等位基因。
