Department of Anesthesiology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.
Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, Chelsea & Westminster Hospital, London, UK.
Theranostics. 2019 Jan 1;9(2):477-490. doi: 10.7150/thno.29361. eCollection 2019.
Diabetic foot ulcers are associated with significant morbidity and mortality, and current treatments are far from optimal. Chronic wounds in diabetes are characterised by impaired angiogenesis, leukocyte function, fibroblast proliferation, and keratinocyte migration and proliferation. : We tested the effect of exposure to argon gas on endothelial cell, fibroblast, macrophage and keratinocyte cell cultures and of a streptozotocin-induced diabetic mouse model. : Exposure to normobaric argon gas promotes multiple steps of the wound healing process. Argon accelerated angiogenesis, associated with upregulation of pro-angiogenic Angiopoietin-1 and vascular endothelial growth factor (VEGF) signalling and . Treatment with argon enhanced expression of transforming growth factor (TGF)-β, early recruitment of macrophages and keratinocyte proliferation. Argon had a pro-survival effect, inducing expression of cytoprotective mediators B-cell lymphoma 2 and heme oxygenase 1. Argon was able to accelerate wound closure in a diabetic mouse model. : Together these findings indicate that argon gas may be a promising candidate for clinical use in treatment of diabetic ulcers.
糖尿病足溃疡与显著的发病率和死亡率相关，而目前的治疗方法远非最佳。糖尿病慢性伤口的特征是血管生成、白细胞功能、成纤维细胞增殖以及角质细胞迁移和增殖受损。我们测试了暴露于氩气对内皮细胞、成纤维细胞、巨噬细胞和角质细胞培养物的影响，以及链脲佐菌素诱导的糖尿病小鼠模型的影响。暴露于常压低氩气促进了伤口愈合过程的多个步骤。氩气加速了血管生成，与上调促血管生成的血管生成素 1 和血管内皮生长因子 (VEGF) 信号有关。氩气治疗增强了转化生长因子 (TGF)-β的表达，早期招募巨噬细胞和角质细胞增殖。氩气具有保护生存的作用，诱导细胞保护介质 B 细胞淋巴瘤 2 和血红素加氧酶 1 的表达。氩气能够加速糖尿病小鼠模型的伤口闭合。这些发现表明，氩气可能是治疗糖尿病溃疡的一种有前途的临床候选药物。
