Nemec Ana, Milevoj Nina, Lampreht Tratar Urša, Serša Gregor, Čemažar Maja, Tozon Nataša
Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
Institute of Oncology Ljubljana, Ljubljana, Slovenia.
Front Vet Sci. 2020 Sep 29;7:575911. doi: 10.3389/fvets.2020.575911. eCollection 2020.
Electroporation is a method of inducing an increase in permeability of the cell membrane through the application of an electric field and can be used as a delivery method for introducing molecules of interest (e.g., chemotherapeutics or plasmid DNA) into cells. Electroporation-based treatments (i.e., electrochemotherapy, gene electrotransfer, and their combinations) have been shown to be safe and effective in veterinary oncology, but they are currently mostly recommended for the treatment of those solid tumors for which clients have declined surgery and/or radiotherapy. Published data show that electroporation-based treatments are also safe, simple, fast and cost-effective treatment alternatives for selected oral and maxillofacial tumors, especially small squamous cell carcinoma and malignant melanoma tumors not involving the bone in dogs. In these patients, a good local response to treatment is expected to result in increased survival time with good quality of life. Despite emerging evidence of the clinical efficacy of electroporation-based treatments for oral and maxillofacial tumors, further investigation is needed to optimize treatment protocols, improve clinical data reporting and better understand the mechanisms of patients' response to the treatment.
电穿孔是一种通过施加电场来增加细胞膜通透性的方法，可作为一种将感兴趣的分子（如化疗药物或质粒DNA）导入细胞的递送方法。基于电穿孔的治疗方法（即电化学疗法、基因电转移及其联合应用）已被证明在兽医肿瘤学中是安全有效的，但目前大多推荐用于治疗那些客户已拒绝手术和/或放疗的实体瘤。已发表的数据表明，基于电穿孔的治疗方法对于选定的口腔颌面部肿瘤，尤其是犬类中不涉及骨骼的小鳞状细胞癌和恶性黑色素瘤肿瘤，也是安全、简单、快速且具有成本效益的治疗选择。在这些患者中，预期良好的局部治疗反应将延长生存时间并提高生活质量。尽管有新证据表明基于电穿孔的治疗方法对口腔颌面部肿瘤具有临床疗效，但仍需要进一步研究以优化治疗方案、改善临床数据报告并更好地理解患者对治疗的反应机制。
