Institute of Polymeric Materials, Sahand University of Technology, Tabriz, Iran.
Faculty of Polymer Engineering, Sahand University of Technology, Tabriz, Iran.
J Biomater Sci Polym Ed. 2021 Mar;32(4):488-503. doi: 10.1080/09205063.2020.1845921. Epub 2020 Nov 16.
To mimic the fibrous architecture of collagen, the nanofibrous gelatin scaffolds are fabricated employing a thermally induced phase separation (TIPS) technique. The influences of processing parameters, including polymer concentration and solvent mixture composition on the scaffold microstructure are investigated. However, using the TIPS technique, a limited pore size range is generally obtained. To yield the well-interconnected macroporous structures with equiaxed pores and nanofibrous architectures, the TIPS technique is combined with particulate leaching. The macroporous structure of produced scaffolds duplicates the predefined three-dimensional template structure. The homogenous macrostructure with well-interconnected equiaxed pores and no particular orientation is created. Modulating the size and shape of microspheres has precise control over porosity, pore size, and interconnection of the matrix. Because of the well-interconnected macroporous nanofibrous structure, the useful applications of these scaffolds in the tissue engineering field are expected.
为了模拟胶原的纤维结构，采用热致相分离（TIPS）技术制备纳米纤维明胶支架。研究了加工参数，包括聚合物浓度和溶剂混合物组成对支架微观结构的影响。然而，使用 TIPS 技术通常只能获得有限的孔径范围。为了获得具有等轴孔和纳米纤维结构的良好连通的大孔结构，将 TIPS 技术与颗粒溶出法结合使用。所制备的支架的大孔结构复制了预定的三维模板结构。形成具有均匀大孔结构、良好连通的等轴孔且无特定取向的结构。通过调节微球的尺寸和形状，可以精确控制基质的孔隙率、孔径和连通性。由于具有良好连通的大孔纳米纤维结构，预计这些支架在组织工程领域有很好的应用。
