School of Public Health, The University of Hong Kong, Pok Fu Lam, Hong Kong Special Administrative Region, China.
Infectious Diseases Centre, Princess Margaret Hospital, Hospital Authority of Hong Kong, Kwai Chung, Hong Kong Special Administrative Region, China.
J Clin Microbiol. 2021 Jan 21;59(2). doi: 10.1128/JCM.02504-20.
Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT) in human, canine, cat, and hamster sera. With PRNT as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT, except for cross-reactivity to SARS-CoV-1 in sVNT.
用于严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的替代中和测定法，如果能够在无需生物安全 3 级防护的情况下在多个物种中进行，将是非常理想的。我们评估了最近开发的替代病毒中和测定法 (sVNT)，并将其与人类、犬科、猫科和仓鼠血清中的 90%蚀斑减少中和测定法 (PRNT) 进行了比较。以 PRNT 作为参考，sVNT 的灵敏度为 98.9%，特异性为 98.8%。使用一组对应于其他冠状病毒的免疫血清，我们证实 SARS-CoV-2 sVNT 和 PRNT 缺乏与其他冠状病毒的交叉反应性，除了 sVNT 与 SARS-CoV-1 的交叉反应性。
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