Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
Parasitology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
Sci Rep. 2020 Nov 2;10(1):18844. doi: 10.1038/s41598-020-74807-1.
Urinary bladder cancer is a common malignancy in Egypt, thus reliable methodologies are required for screening and early detection. In this study, we analyzed the gene expression of a Schistosoma hematobium specific microRNA "Sha-miR-71a" and mitogen-associated protein kinase-3 (MAPK-3) in the urine samples of 50 bladder cancer patients and 50 patients with benign bilharzial cystitis. Fifty control subjects were also tested. Indirect hemagglutination test (IHA) diagnosed 70% of studied cancer cases as bilharzial associated bladder cancer (BBC), while histopathological examination detected only 18%. Urinary Sha-miR-71a & MAPK-3 revealed enhanced expression in BBC (p-value = 0.001) compared to non-bilharzial bladder cancer (NBBC) cases. Patients with chronic bilharzial cystitis exhibited a significant increase in gene expression compared to those with acute infection (p-value = 0.001). Sha-miR-71a and MAPK-3 showed good sensitivity and specificity in the diagnosis of BBC when analyzed by the receiver operating characteristic (ROC) curve. They were also prognostic regarding malignancy grade. Both biomarkers showed a positive correlation. Our results revealed that IHA is a reliable test in the diagnosis of bilharziasis associated with bladder cancer, and that Sha-miR-71a and MAPK-3 provide non-invasive specific biomarkers to diagnose BBC, as well as a potential role in testing bilharzial patients for risk to develop cancer.
膀胱癌是埃及常见的恶性肿瘤，因此需要可靠的方法进行筛查和早期检测。在这项研究中，我们分析了埃及血吸虫特异性 microRNA“Sha-miR-71a”和丝裂原活化蛋白激酶-3（MAPK-3）在 50 例膀胱癌患者和 50 例良性埃及血吸虫性膀胱炎患者尿液样本中的基因表达。还对 50 名对照进行了测试。间接血凝试验（IHA）将 70%的研究癌症病例诊断为埃及血吸虫相关性膀胱癌（BBC），而组织病理学检查仅发现 18%。与非埃及血吸虫性膀胱癌（NBBC）病例相比，BBC 中尿 Sha-miR-71a 和 MAPK-3 的表达明显增强（p 值=0.001）。与急性感染相比，慢性埃及血吸虫性膀胱炎患者的基因表达显著增加（p 值=0.001）。通过接收者操作特征（ROC）曲线分析，Sha-miR-71a 和 MAPK-3 在 BBC 的诊断中具有良好的敏感性和特异性。它们在恶性程度方面也具有预后价值。这两种生物标志物呈正相关。我们的结果表明，IHA 是诊断与膀胱癌相关的埃及血吸虫病的可靠试验，Sha-miR-71a 和 MAPK-3 提供了非侵入性的特异性生物标志物来诊断 BBC，并可能在检测埃及血吸虫病患者患癌症的风险方面发挥作用。
