Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.
College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
Arch Microbiol. 2021 Apr;203(3):1039-1045. doi: 10.1007/s00203-020-02097-2. Epub 2020 Nov 2.
A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. The optimal temperature and pH for the growth of strain LAM7117 were 35 °C and 7.5, respectively. Strain LAM7117 could grow in the presence of NaCl with concentration up to 9% (w/v). Strain LAM7117 formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522 (97.7%). The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117 and A. crystallopoietes JCM 2522 were 21.4 and 77.4%, respectively. The genomic DNA G + C content was 65.9 mol%. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid. The major polar lipids present in strain LAM7117 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid. The predominant menaquinones of strain LAM7117 were MK-8 and MK-9. Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117 should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp. nov. is proposed. The type strain is LAM7117 (= JCM 32824 = CGMCC 1.16681).
一株革兰氏阳性、需氧、能运动的杆菌，被命名为 LAM7117 菌株，是从富含桦木林土壤的磺酰脲类除草剂降解菌富集物中分离得到的。LAM7117 菌株的最适生长温度和 pH 值分别为 35°C 和 7.5。该菌株可以在含有高达 9%（w/v）NaCl 的环境中生长。在基于 16S rRNA 基因序列构建的系统发育树中，LAM7117 菌株在节杆菌属内形成了一个独特的系统发育亚群，与 A. crystallopoietes JCM 2522 的相似度最高（97.7%）。根据基因组序列计算的 LAM7117 与 A. crystallopoietes JCM 2522 之间的数字 DNA-DNA 同源性和 Avery 核苷酸同一性值分别为 21.4%和 77.4%。基因组 DNA G+C 含量为 65.9 mol%。主要的细胞脂肪酸为 anteiso-C、iso-C 和 anteiso-C。细胞壁肽聚糖含有甘氨酸、赖氨酸、丙氨酸和谷氨酸。LAM7117 中存在的主要极性脂为二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、两种未鉴定的糖脂和一种未鉴定的脂。该菌株的主要menaquinones 为 MK-8 和 MK-9。基于表型特征、化学分类学数据和基因型分析，LAM7117 菌株应归类为节杆菌属的一个新种，命名为磺酰脲降解菌节杆菌（Arthrobacter sulfonylureivorans）sp. nov.。模式菌株为 LAM7117（=JCM 32824=CGMCC 1.16681）。
