Veterinary Diagnostic Laboratory and Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN.
Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, Egypt.
J Vet Diagn Invest. 2020 Nov;32(6):873-879. doi: 10.1177/1040638720949475. Epub 2020 Aug 17.
Kobuvirus infections are common among humans, rodents, carnivores, pigs, and ruminants. We report herein the complete genome sequence of a novel caprine kobuvirus (MN604700) from diarrheic kids in Minnesota. Whole-genome sequencing revealed a kobuvirus genome of 8,139 nt with a single ORF region encoding a polyprotein of 2,480 amino acids. Further analysis revealed nt substitutions along the genome compared with that of the caprine kobuvirus reference strain, with 93% identity. Phylogenetic analysis indicated that the clade of the caprine kobuvirus was most closely related to porcine kobuviruses rather than bovine or ovine kobuviruses. Using primers designed from this genome, caprine kobuvirus was identified in the stools of other goats. Sanger sequencing of PCR products indicated and gene nucleotides of this latter strain were 95% and 91% identical with those of MN604700, respectively. There were 35 and 101 nt substitutions in and genes, respectively. Findings of kobuvirus over a 2-y period may indicate an endemic state, which needs further research. In addition, screening for kobuviruses over large geographic areas is needed to identify the evolutionary connections among different strains.
科博病毒感染在人类、啮齿动物、食肉动物、猪和反刍动物中很常见。我们在此报告了一种新型绵羊科博病毒（MN604700）的完整基因组序列，该病毒来自明尼苏达州腹泻的幼崽。全基因组测序显示，科博病毒基因组长 8139 个核苷酸，具有一个单一的 ORF 区域，编码 2480 个氨基酸的多蛋白。进一步分析显示，与绵羊科博病毒参考株相比，基因组中有核苷酸取代，同源性为 93%。系统发育分析表明，绵羊科博病毒的分支与猪科博病毒最为密切相关，而不是牛科或羊科科博病毒。使用从该基因组设计的引物，在其他山羊的粪便中鉴定出了绵羊科博病毒。PCR 产物的 Sanger 测序表明，该病毒株的 和 基因核苷酸分别与 MN604700 有 95%和 91%的同源性。 和 基因分别有 35 个和 101 个核苷酸取代。在 2 年的时间里发现科博病毒可能表明存在地方性流行状态，这需要进一步研究。此外，需要在更大的地理区域内筛选科博病毒，以确定不同菌株之间的进化联系。
