Lu Lu, Van Dung Nguyen, Ivens Alasdair, Bogaardt Carlijn, O'Toole Aine, Bryant Juliet E, Carrique-Mas Juan, Van Cuong Nguyen, Anh Pham Hong, Rabaa Maia A, Tue Ngo Tri, Thwaites Guy E, Baker Stephen, Simmonds Peter, Woolhouse Mark Ej
Ashworth Laboratories, Centre for Immunity, Infection and Evolution, University of Edinburgh, Kings Buildings, Charlotte Auerbach Road, Edinburgh EH9 3FL, UK.
Nuffield Department of Medicine, University of Oxford, South Parks Road, Oxford OX1 3SY, UK.
Virus Evol. 2018 Feb 13;4(1):vey002. doi: 10.1093/ve/vey002. eCollection 2018 Jan.
Cross-species transmission of viruses poses a sustained threat to public health. Due to increased contact between humans and other animal species the possibility exists for cross-species transmissions and ensuing disease outbreaks. By using conventional PCR amplification and next generation sequencing, we obtained 130 partial or full genome kobuvirus sequences from humans in a sentinel cohort in Vietnam and various mammalian hosts including bats, rodents, pigs, cats, and civets. The evolution of kobuviruses in different hosts was analysed using Bayesian phylogenetic methods. We estimated and compared time of origin of kobuviruses in different host orders; we also examined the cross-species transmission of kobuviruses within the same host order and between different host orders. Our data provide new knowledge of rodent and bat kobuviruses, which are most closely related to human kobuviruses. The novel bat kobuviruses isolated from bat roosts in Southern Vietnam were genetically distinct from previously described bat kobuviruses, but closely related to kobuviruses found in rodents. We additionally found evidence of frequent cross-species transmissions of kobuviruses within rodents. Overall, our phylogenetic analyses reveal multiple cross-species transmissions both within and among mammalian species, which increases our understanding of kobuviruses genetic diversity and the complexity of their evolutionary history.
病毒的跨物种传播对公共卫生构成持续威胁。由于人类与其他动物物种之间的接触增加，存在跨物种传播及随之而来的疾病暴发的可能性。通过使用常规PCR扩增和下一代测序技术，我们从越南一个哨点队列中的人类以及包括蝙蝠、啮齿动物、猪、猫和果子狸在内的各种哺乳动物宿主中获得了130条部分或完整基因组的杯状病毒序列。使用贝叶斯系统发育方法分析了不同宿主中杯状病毒的进化情况。我们估计并比较了不同宿主目中杯状病毒的起源时间；我们还研究了杯状病毒在同一宿主目内以及不同宿主目之间的跨物种传播。我们的数据提供了与人类杯状病毒关系最为密切的啮齿动物和蝙蝠杯状病毒的新知识。从越南南部蝙蝠栖息地分离出的新型蝙蝠杯状病毒在基因上与先前描述的蝙蝠杯状病毒不同，但与在啮齿动物中发现的杯状病毒密切相关。我们还发现了杯状病毒在啮齿动物中频繁跨物种传播的证据。总体而言，我们的系统发育分析揭示了哺乳动物物种内部和之间的多次跨物种传播，这增加了我们对杯状病毒遗传多样性及其进化历史复杂性的理解。
