Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2021 Feb 28;31(2):250-258. doi: 10.4014/jmb.2009.09017.
Among various species of marine bacteria, those belonging to the genus have several promising applications and have been studied well. However, not much information has been available on their antibiotic resistance. In our efforts to learn about the antibiotic resistance of strain CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin differed in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying this NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions while also performing time-dependent reverse transcription PCR. We found that the gene encoding aminoglycoside phosphotransferase showed increased expression under the 10% rather than 1% NaCl conditions. When these genes were overexpressed in an strain, pETDuet-1:: showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control, suggesting was involved in aminoglycoside resistance. Our results demonstrated a more direct link between NaCl and aminoglycoside resistance exhibited by the CKY01 strain.
在各种海洋细菌中，属于 的那些具有几种有前途的应用，并已得到很好的研究。然而，关于它们的抗生素耐药性的信息并不多。在我们努力了解先前研究中表现出产生各种水解酶和渗透剂促进生长的菌株 CKY01 的抗生素耐药性时，我们发现它对包括卡那霉素、氨苄西林、苯唑西林、羧苄西林、庆大霉素、阿普拉霉素、四环素和大观霉素在内的多种抗生素表现出耐药性。然而，在培养基中存在 10% NaCl 和 1% NaCl 时，CKY01 对卡那霉素、庆大霉素、阿普拉霉素、四环素和大观霉素的耐药模式不同。为了确定这种 NaCl 浓度依赖性抗生素耐药性的机制，我们比较了不同盐条件下的四个氨基糖苷类耐药基因，同时进行了时间依赖性逆转录 PCR。我们发现，编码氨基糖苷磷酸转移酶的 基因在 10%而不是 1% NaCl 条件下表达增加。当这些基因在 菌株中过表达时，pETDuet-1:: 在存在卡那霉素、庆大霉素和阿普拉霉素时显示出比各自对照更小的抑制区，表明 参与了氨基糖苷类抗生素的耐药性。我们的结果表明，CKY01 菌株中 NaCl 和氨基糖苷类抗生素耐药性之间存在更直接的联系。
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