适配体修饰的纸质电化学器件的现场生物传感制造。
Fabrication of Aptamer-Modified Paper Electrochemical Devices for On-Site Biosensing.
机构信息
Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th St, Miami, FL, 33199, USA.
出版信息
Angew Chem Int Ed Engl. 2021 Feb 8;60(6):2993-3000. doi: 10.1002/anie.202008231. Epub 2020 Dec 10.
Abstract
Electrochemical aptamer-based (E-AB) sensors offer a powerful and general means for analyte detection in complex samples for various applications. Paper-based E-AB sensors could enable portable, low-cost, and rapid detection of a broad range of targets, but it has proven challenging to fabricate suitable three-electrode systems on paper. Here, we demonstrate a simple, economic, and environmentally friendly strategy for fabricating aptamer-modified paper electrochemical devices (PEDs) via ambient vacuum filtration. The material, shape, size, and thickness of the three-electrode PED system can be fully customized. We developed aptamer-modified PEDs that enable sensitive and specific detection of small molecules in minimally processed biosamples. The sensitivity and stability of the PEDs are comparable to E-AB sensors based on commercial gold electrodes. We believe our strategy can lead to the development of high performance PEDs for the on-site detection of a variety of analytes.
摘要
基于电化学适体的（E-AB）传感器为各种应用中复杂样品中的分析物检测提供了一种强大而通用的手段。基于纸张的 E-AB 传感器可以实现便携式、低成本和快速检测广泛的目标，但在纸张上制造合适的三电极系统一直具有挑战性。在这里，我们展示了一种简单、经济且环保的策略，通过环境真空过滤在纸张上制造适体修饰的纸电化学器件（PED）。三电极 PED 系统的材料、形状、尺寸和厚度可以完全定制。我们开发了适体修饰的 PED，可以对经过最小化处理的生物样品中的小分子进行灵敏和特异性检测。PED 的灵敏度和稳定性与基于商业金电极的 E-AB 传感器相当。我们相信我们的策略可以为各种分析物的现场检测开发高性能的 PED。
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