Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; Shandong Provincial Key Laboratory of Biosensors, Jinan 250103, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
Int J Biol Macromol. 2021 Jan 1;166:1220-1229. doi: 10.1016/j.ijbiomac.2020.11.004. Epub 2020 Nov 4.
The aim of this study was to investigate sulfuric acid degradation of the Pholiota nameko polysaccharide (AIPS-1). Three stepwise degraded polysaccharides (AIPS-2, AIPS-3, and AIPS-4) were obtained by sequentially increasing the strength of sulfuric acid treatment. Structural characterization showed that sulfuric acid treatment significantly decreased molecular weight, increased the content of uronic acid and changed the molar ratio of monosaccharide composition, while the major functional groups and the triple helical conformation of polysaccharides did not change significantly. In vitro experiments proved that the antioxidation ability of the stepwise degraded polysaccharides gradually increased (AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4). An oxidative stress zebrafish model was established, which demonstrated that the ability of AIPS-3 and AIPS-4 to scavenge free radicals in zebrafish was significantly improved compared to AIPS-1. In conclusion, sulfuric acid treatment is an effective method for improving the antioxidant activity of polysaccharides, and increased antioxidant activity was closely related to the changes in their structural characteristics.
本研究旨在探讨硫酸对香菇多糖（AIPS-1）的降解作用。通过逐步增加硫酸处理的强度，得到了三种逐步降解的多糖（AIPS-2、AIPS-3 和 AIPS-4）。结构表征表明，硫酸处理显著降低了分子量，增加了酸性多糖的含量，并改变了单糖组成的摩尔比，而多糖的主要功能基团和三螺旋构象没有发生明显变化。体外实验证明，逐步降解多糖的抗氧化能力逐渐增强（AIPS-1 < AIPS-2 < AIPS-3 < AIPS-4）。建立了氧化应激斑马鱼模型，结果表明，与 AIPS-1 相比，AIPS-3 和 AIPS-4 清除斑马鱼自由基的能力显著提高。综上所述，硫酸处理是提高多糖抗氧化活性的有效方法，而抗氧化活性的提高与它们结构特征的变化密切相关。
