Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
Department of Microbiology, Faculty of Medicine, Rabigh Branch, King Abdulaziz University, Saudi Arabia.
Complement Ther Clin Pract. 2020 Nov;41:101257. doi: 10.1016/j.ctcp.2020.101257. Epub 2020 Nov 2.
The health benefits of honey as an oral therapeutic agent for the treatment of diarrhoea caused by Shigella sonnei depend on the ability of honey to withstand human gastrointestinal conditions. This study aimed to investigate whether honey could withstand and inhibit the growth of Shigella sonnei under such conditions.
We initially evaluated the survival of Shigella sonnei in human simulated gastric conditions (SGC) and simulated intestinal conditions (SIC). This was followed by determination of the susceptibility of Shigella sonnei to Manuka and Talah honey under gastrointestinal conditions. The colony forming units (CFU) of Shigella sonnei and minimum inhibitory concentrations (MICs) of honey were calculated.
Shigella sonnei was unable to survive in the acidic environment of the stomach without food matrix and survived only when inoculated with a food source, resulting in 1.5 × 10 ± 0.2 CFU at 60 min and 1.7 × 10 ± 0.3 CFU after 120 min of incubation. In SIC, it survived both with and without food matrix at the same CFU (1.2 × 10 ±0.4) at 60 min and 1.7 × 10 ±0.2 CFU after 120 min of incubation. Growth of Shigella sonnei was not observed in SGC in the presence of either honey at different concentrations without a food source. In the presence of a food source, Manuka honey inhibited the growth of Shigella sonnei at 10% v/v and Talah honey at 20% v/v dilutions in SGC. In SIC, Manuka honey inhibited the growth of Shigella sonnei at 15% and 20% v/v dilutions, whereas Talah honey inhibited Shigella sonnei at 20% and 25% v/v dilutions without and with food sources, respectively.
Shigella sonnei can survive in the acidic environment of the stomach if inoculated with a food source. Acidic pH and pepsin had no deleterious effects on the antibacterial capability of honey. However, bile reduced the antibacterial activity of honey in the intestinal environment.
蜂蜜作为一种口服治疗剂，对志贺氏菌引起的腹泻具有健康益处，这取决于蜂蜜在人体胃肠道条件下的生存能力。本研究旨在探讨蜂蜜在这些条件下是否能够生存并抑制志贺氏菌的生长。
我们首先评估了志贺氏菌在人模拟胃条件（SGC）和模拟肠条件（SIC）下的生存能力。随后，我们测定了在胃肠道条件下，麦卢卡和塔拉蜂蜜对志贺氏菌的敏感性。计算了志贺氏菌的菌落形成单位（CFU）和蜂蜜的最小抑菌浓度（MIC）。
志贺氏菌在没有食物基质的酸性胃液环境中无法存活，只有在接种食物源时才能存活，在 60 分钟时培养物中的 CFU 为 1.5×10±0.2，在 120 分钟时培养物中的 CFU 为 1.7×10±0.3。在 SIC 中，无论是否有食物基质，志贺氏菌在 60 分钟时的 CFU 均相同（1.2×10±0.4），在 120 分钟时的 CFU 为 1.7×10±0.2。在没有食物源的情况下，不同浓度的蜂蜜均能抑制 SGC 中志贺氏菌的生长。在有食物源的情况下，10%v/v 的麦卢卡蜂蜜和 20%v/v 的塔拉蜂蜜抑制 SGC 中志贺氏菌的生长。在 SIC 中，15%和 20%v/v 的麦卢卡蜂蜜和 20%和 25%v/v 的塔拉蜂蜜抑制有和无食物源的志贺氏菌生长。
如果接种食物源，志贺氏菌可以在胃酸环境中存活。酸性 pH 值和胃蛋白酶对蜂蜜的抗菌能力没有有害影响。然而，胆汁降低了蜂蜜在肠道环境中的抗菌活性。
