维生素 E 水平变化作为女性不孕的标志物。

Changes in vitamin E levels as a marker of female infertility.

机构信息

Department of Biological and Biomedical Sciences, University of Karachi, Karachi, Pakistan.

Graduate Student 2019, University of Karachi, Karachi, Pakistan.

出版信息

J Pak Med Assoc. 2020 Oct;70(10):1762-1766. doi: 10.5455/JPMA.40329.

DOI:10.5455/JPMA.40329

PMID:33159749

Abstract

OBJECTIVE

To study the impact of Vitamin E (VE) levels of follicular fluid (FF) on oocyte competence, embryo development and pregnancy outcome in patients after intra cytoplasmic sperm injection (ICSI).

METHODS

It was a cross-sectional study conducted in Islamabad Clinic Serving Infertile Couples in which follicular fluid of 137 females booked for ICSI, was obtained during oocyte retrieval, centrifuged and stored for analysis. VE levels in FF were analyzed by enzyme linked immune sorbent assay. Receiver Operating Curve (ROC)was used to demarcate VE levels required for acquiring pregnancy. Generalized linear model using log binomial regression was applied to see the effect of VE on pregnancy, the effect of VE on oocyte and embryo parameters was assessed by linear regression; all p-values less than 0.05 were considered statistically significant.

RESULTS

ROC suggested 5.49 (unit) as the cutoff value of VE in the pregnancy group, with 72.9% area under the curve. Ninety-one females comprised Group I with VE > 5.49, whereas forty six females formed Group II with VE < 5.49. Follicular fluid VE levels were significantly high in 39 (28.5%) females who compromised pregnancy group. Chances of pregnancy increased to 4% with an increase in VE levels (p-value 0.01). VE gave significant positive relationship with all oocyte (retrieved, mature and fertilized) parameters, cleavage of embryo till its differentiation to blastocysts (p<0.01).

CONCLUSIONS

Adequate amount of VE in follicular fluid enhances the possibility of maturation of oocytes which resulted in better reproductive outcome after ICSI.

摘要

目的

研究卵泡液（FF）中的维生素 E（VE）水平对接受胞浆内单精子注射（ICSI）的患者的卵母细胞能力、胚胎发育和妊娠结局的影响。

方法

这是一项在伊斯兰堡诊所进行的横断面研究，该诊所为不孕夫妇提供服务，在卵母细胞回收期间从 137 名接受 ICSI 的女性中获得卵泡液，离心后储存进行分析。采用酶联免疫吸附试验分析 FF 中的 VE 水平。使用接收者操作特征曲线（ROC）来划定获得妊娠所需的 VE 水平。应用对数二项式回归广义线性模型来观察 VE 对妊娠的影响，用线性回归来评估 VE 对卵母细胞和胚胎参数的影响；所有 p 值小于 0.05 均被认为具有统计学意义。

结果

ROC 提示 VE 的截断值为 5.49（单位），曲线下面积为 72.9%。91 名女性组成 VE > 5.49 的组 I，46 名女性组成 VE < 5.49 的组 II。在导致妊娠组的 39 名女性（28.5%）中，卵泡液 VE 水平明显较高。VE 水平每增加 1%，妊娠的机会增加 4%（p 值 0.01）。VE 与所有卵母细胞（回收、成熟和受精）参数以及胚胎分裂至囊胚分化（p<0.01）呈显著正相关。