Université de Paris, CNRS, Institut Jacques Monod, F-75006, Paris, France.
Curr Opin Cell Biol. 2021 Feb;68:72-80. doi: 10.1016/j.ceb.2020.09.002. Epub 2020 Oct 23.
In cells, the actin cytoskeleton is regulated by an interplay between mechanics and biochemistry. A key mechanism, which has emerged based on converging indications from structural, cellular, and biophysical data, depicts the actin filament as a mechanically tunable substrate: mechanical stress applied to an actin filament induces conformational changes, which modify the binding and the regulatory action of actin-binding proteins. For a long time, however, direct evidence of this mechanotransductive mechanism was very scarce. This situation is changing rapidly, and recent in vitro single-filament studies using different techniques have revealed that several actin-binding proteins are able to sense tension, curvature, and/or torsion, applied to actin filaments. Here, we discuss these recent advances and their possible implications.
在细胞中，肌动蛋白细胞骨架通过力学和生物化学之间的相互作用进行调节。一个关键的机制，根据结构、细胞和生物物理数据的综合迹象已经出现，描述了肌动蛋白丝作为一种机械可调底物：施加在肌动蛋白丝上的机械应力诱导构象变化，从而改变肌动蛋白结合蛋白的结合和调节作用。然而，很长一段时间以来，这种机械转导机制的直接证据非常缺乏。这种情况正在迅速改变，最近使用不同技术的体外单丝研究表明，几种肌动蛋白结合蛋白能够感知施加在肌动蛋白丝上的张力、曲率和/或扭转。在这里，我们讨论这些最新进展及其可能的影响。
