苏丹恶性疟原虫抗药性标志物的快照：一项初步研究。
A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study.
机构信息
Department of Molecular Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile University, Khartoum, Sudan.
Department of Biotechnology, School of Pharmacy, Ahfad University for Women, Omdurman, Sudan.
出版信息
BMC Res Notes. 2020 Nov 7;13(1):512. doi: 10.1186/s13104-020-05363-0.
OBJECTIVES
Malaria infection is still known to be a worldwide public health problem, especially in tropical and sub-tropical African countries like Sudan. A pilot study conducted to describe the trend of P. falciparum drug resistance markers in 2017-2018 in comparison to CQ and AS/SP eras in Sudan. The Pfcrt, Pfmdr-1, Pfdhfr, and Pfdhps genes were investigated. Data deposited by the worldwide antimalarial resistance network was consulted, and the molecular markers previously reported from Sudan were analyzed.
RESULTS
Drug molecular markers analysis was successfully done on 20 P. falciparum isolates. The Pfcrt K76 showed high frequency; 16 (80%). For the Pfmdr-1, 9 (45%) isolates were carrying the N86 allele, and 11 (55%) were 86Y allele. While the Y184F of the Pfmdr-1 showed a higher frequency of 184F compared to Y184; 16 (80%) and 4 (20%), respectively. In the Pfdhfr, 51I allele showed higher frequency compared to N51; 18 (90%) and 2 (10%), respectively. For S108N, 18 (90%) were 108 N and 2 (10%) were S108. In the Pfdhps, all isolates were carrying the mutant alleles; 437G and 540E. The frequency distribution of the Pfcrt, Pfmdr-1, Pfdhfr, Pfdhps was significantly different across the whole years in Sudan.
目的
疟疾感染仍然是一个全球性的公共卫生问题，特别是在像苏丹这样的热带和亚热带非洲国家。本研究旨在描述 2017-2018 年在苏丹流行的恶性疟原虫耐药性标志物的趋势，以与氯喹和磺胺多辛-乙胺嘧啶时期进行比较。研究检测了 Pfcrt、Pfmdr-1、Pfdhfr 和 Pfdhps 基因。咨询了全球抗疟耐药网络的数据，分析了之前在苏丹报道的分子标志物。
结果
成功分析了 20 株恶性疟原虫分离株的药物分子标志物。Pfcrt K76 表现出高频，16 株（80%）。Pfmdr-1 的 N86 等位基因携带率为 9 株（45%），86Y 等位基因携带率为 11 株（55%）。而 Pfmdr-1 的 Y184F 比 Y184 更常见，16 株（80%）和 4 株（20%）。在 Pfdhfr 中，51I 等位基因比 N51 更常见，18 株（90%）和 2 株（10%）。S108N 中有 18 株（90%）为 108N，2 株（10%）为 S108。在 Pfdhps 中，所有分离株均携带突变等位基因 437G 和 540E。在苏丹，Pfcrt、Pfmdr-1、Pfdhfr、Pfdhps 的频率分布在整个年份都有显著差异。
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