Anderson J M, Soll D R
Department of Biology, University of Iowa, Iowa City 52242.
J Bacteriol. 1987 Dec;169(12):5579-88. doi: 10.1128/jb.169.12.5579-5588.1987.
Select strains of Candida albicans switch reversibly and at extremely high frequency between a white and an opaque colony-forming phenotype, which has been referred to as the white-opaque transition. Cells in the white phase exhibit a cellular phenotype indistinguishable from that of most standard strains of C. albicans, but cells in the opaque phase exhibit an unusually large, elongate cellular shape. In comparing the white and opaque cellular phenotypes, the following findings are demonstrated. (i) The surface of the cell wall of maturing opaque cells when viewed by scanning electron microscopy exhibits a unique pimpled, or punctate, pattern not observed in white cells or standard strains of C. albicans. (ii) The dynamics of actin localization which accompanies opaque-cell growth first follows the pattern of budding cells during early opaque-bud growth and then the pattern of hypha-forming cells during late opaque-bud growth. (iii) A hypha-specific cell surface antigen is also expressed on the surface of opaque budding cells. (iv) An opaque-specific surface antigen is distributed in a punctate pattern.
白色念珠菌的某些菌株会在白色和不透明菌落形成表型之间以极高频率进行可逆转换，这一现象被称为白-不透明转换。白色阶段的细胞表现出与大多数白色念珠菌标准菌株难以区分的细胞表型，但不透明阶段的细胞呈现出异常大且细长的细胞形态。在比较白色和不透明细胞表型时，有以下发现：(i) 通过扫描电子显微镜观察，成熟不透明细胞的细胞壁表面呈现出独特的丘疹状或点状图案，而在白色细胞或白色念珠菌标准菌株中未观察到。(ii) 伴随着不透明细胞生长的肌动蛋白定位动态变化，在不透明芽早期生长时首先遵循出芽细胞的模式，然后在不透明芽后期生长时遵循形成菌丝细胞的模式。(iii) 一种菌丝特异性细胞表面抗原也在不透明芽细胞表面表达。(iv) 一种不透明特异性表面抗原以点状模式分布。
