Rikkerink E H, Magee B B, Magee P T
Department of Microbiology, Michigan State University, East Lansing 48824-1101.
J Bacteriol. 1988 Feb;170(2):895-9. doi: 10.1128/jb.170.2.895-899.1988.
This paper reports that the opaque and white phenotypes of Candida albicans constitute a true high-frequency reversible transition system. The rDNA restriction fragment and orthogonal field alternating gel electrophoresis profiles of opaque and white phenotypes are indistinguishable, and a genetic marker introduced into a white strain is present in all opaque derivatives of this strain. Opaque and white derivatives appear markedly different on a bismuth indicator medium and differ in a number of other respects. We have used bismuth medium to examine the spontaneous and temperature-induced frequencies of transition from opaque to white. The temperature-induced transition from opaque to white does not occur when opaque cells are held in water.
本文报道白色念珠菌的不透明和白色表型构成一个真正的高频可逆转变系统。不透明和白色表型的rDNA限制性片段和正交场交变凝胶电泳图谱无法区分，导入白色菌株的遗传标记存在于该菌株的所有不透明衍生物中。不透明和白色衍生物在铋指示培养基上表现出明显差异，在其他许多方面也有所不同。我们利用铋培养基检测从不透明到白色的自发和温度诱导转变频率。当不透明细胞保存在水中时，不会发生温度诱导的从不透明到白色的转变。
