Meng Tianjiao, Shang Ningzhao, Nsabimana Anaclet, Ye Huimin, Wang Huan, Wang Chun, Zhang Yufan
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Hebei University, 071002, Baoding, PR China.
College of Science, Hebei Agricultural University, 071001, Baoding, PR China.
Anal Chim Acta. 2020 Nov 22;1138:59-68. doi: 10.1016/j.aca.2020.09.022. Epub 2020 Sep 12.
MicroRNA-21 (miR-21) has been widely investigated as important biomarkers for cancer diagnosis and treatment. Herein, a highly sensitive nonenzymatic electrochemical biosensor based on Pd@metal-organic frameworks (Pd@UiO-66) and target-catalytic hairpin assembly (CHA) with target recycling approach has been proposed for the detection of miR-21. The proposed biosensor integrates the efficient CHA strategy and excellent electrocatalytic performance of Pd@UiO-66 nanocomposites. The concentration of miRNA-21 is related to the amount of the adsorbed electrocatalyst, leading to the different electrochemical signals for readout towards paracetamol (AP). This biosensor shows a low limit of detection of 0.713 fM with the dynamic range of 20 fM -600 pM under the optimal experimental conditions, providing a powerful platform for detecting miR-21. Furthermore, the designed biochemical self-assembly strategy of this electrochemical biosensor is promising candidate for potential applications in the analysis of other important genetic biomarkers for early diagnosis of cancers.
微小RNA-21（miR-21）作为癌症诊断和治疗的重要生物标志物已被广泛研究。在此，我们提出了一种基于钯@金属有机框架（Pd@UiO-66）和具有靶标循环方法的靶标催化发夹组装（CHA）的高灵敏度非酶电化学生物传感器，用于检测miR-21。所提出的生物传感器整合了高效的CHA策略和Pd@UiO-66纳米复合材料优异的电催化性能。miRNA-21的浓度与吸附的电催化剂的量有关，从而导致对乙酰氨基酚（AP）读出的不同电化学信号。在最佳实验条件下，该生物传感器的检测限低至0.713 fM，动态范围为20 fM - 600 pM，为检测miR-21提供了一个强大的平台。此外，这种电化学生物传感器设计的生化自组装策略有望成为用于癌症早期诊断的其他重要遗传生物标志物分析的潜在应用候选方案。
