Amang A P, Kodji E, Mezui C, Baane M P, Siwe G T, Kuissu T M, Emakoua J, Tan P V
Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.
Evid Based Complement Alternat Med. 2020 Oct 24;2020:6297475. doi: 10.1155/2020/6297475. eCollection 2020.
is a plant used in Cameroonian ethnomedicine to cure jaundice. The aim of this study was to evaluate the hepatoprotective activity of leaves aqueous extract in rats subjected to ethanol-induced liver damage. . 36 rats including 18 males and 18 females were divided into 6 groups of 3 males and 3 females each, namely, 3 control groups (normal, negative, positive) and 3 test groups. The normal and negative control groups were given distilled water (5 ml/kg), the positive control group received silymarin (100 mg/kg), and the test groups were given extract at the doses of 100, 200, and 400 mg/kg. All groups, except the normal control, received concomitantly and daily 40% ethanol (4 g/kg) for 3 weeks to induce hepatotoxicity. Biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, and lipid profile (total cholesterol, HDL, LDL, and triglycerides) were evaluated. Histological sections of the liver, kidneys, and lungs were examined. Qualitative and quantitative phytochemical analysis of the extract were carried out.
The groups treated with the extract at the doses of 200 and 400 mg/kg showed a significant decrease ( < 0.001) of transaminases (ALT and AST), ALP, and bilirubin compared with the negative control. These results were confirmed by observation of histological sections of the liver that confirmed protective action of extract against ethanol-induced hepatocellular injury.
possesses hepatoprotective properties that could be related to its high content of tannins and saponins in the leaves aqueous extract.
是一种用于喀麦隆民族医学治疗黄疸的植物。本研究的目的是评估叶水提取物对乙醇诱导的肝损伤大鼠的肝保护活性。36只大鼠，包括18只雄性和18只雌性，被分为6组，每组3只雄性和3只雌性，即3个对照组（正常、阴性、阳性）和3个试验组。正常对照组和阴性对照组给予蒸馏水（5毫升/千克），阳性对照组给予水飞蓟素（100毫克/千克），试验组给予提取物，剂量分别为100、200和400毫克/千克。除正常对照组外，所有组每天同时给予40%乙醇（4克/千克），持续3周以诱导肝毒性。评估了丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、胆红素和血脂谱（总胆固醇、高密度脂蛋白、低密度脂蛋白和甘油三酯）等生化参数。检查了肝脏、肾脏和肺的组织切片。对提取物进行了定性和定量植物化学分析。
与阴性对照组相比，给予200和400毫克/千克剂量提取物治疗的组转氨酶（ALT和AST）、ALP和胆红素显著降低（<0.001）。肝脏组织切片的观察证实了提取物对乙醇诱导的肝细胞损伤的保护作用，从而证实了这些结果。
具有肝保护特性，这可能与其叶水提取物中高含量的单宁和皂苷有关。
