Dahiru D, Obidoa O
Department of Biochemistry, Federal University of Technology, Yola P.M.B. 2076 Yola, Adamawa State, Nigeria. v
Afr J Tradit Complement Altern Med. 2007 Oct 27;5(1):39-45. doi: 10.4314/ajtcam.v5i1.31254.
Chronic alcohol ingestion is known to increase the generation of reactive oxygen species (ROS), thereby leading to liver damage. Antioxidant enzymes act individually or in combination to reduce or counter the effect of these ROS. Chronic administration of alcohol at (40% v/v, 1 ml/100 g), for 6 weeks showed a significant (p<0.05) elevated levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TB). There was also a significant (p<0.05) decreased levels of catalase, glutathione peroxidase, glutathione reductase and superoxide dismutase compared to control rats. Pre-treatment of rats with 200, 400 mg/kg body weight of aqueous leaf extract of Ziziphus mauritiana or 100 mg/kg silymarin resulted in a significant (p<0.05) decreased levels of ALT, AST, ALP, and TB with levels of catalase, glutathione peroxidase, glutathione reductase and superoxide dismutase showing a significant (p<0.05) increase compared to group administered alcohol only. Histopathology of rat liver administered with alcohol only resulted in severe necrosis, mononuclear cell aggregation and fatty degeneration in the central and mid zonal areas which was a characteristic of a damaged liver. Pre-treatment with the aqueous extract of Ziziphus mauritiana or silymarin reduced the morphological changes that are associated with chronic alcohol administration. The presence of tannins, saponins and phenolic compounds observed in the plant extract could be responsible for the observed effects of decreasing the levels of injured tissue marker and lipid peroxidation.
已知长期摄入酒精会增加活性氧（ROS）的生成，从而导致肝损伤。抗氧化酶单独或联合发挥作用，以减少或对抗这些ROS的影响。以40%（体积/体积）、1毫升/100克的剂量对大鼠进行6周的酒精慢性给药后，丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）和总胆红素（TB）水平显著升高（p<0.05）。与对照大鼠相比，过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和超氧化物歧化酶的水平也显著降低（p<0.05）。用200、400毫克/千克体重的毛里求斯枣树叶水提取物或100毫克/千克水飞蓟宾对大鼠进行预处理，导致ALT、AST、ALP和TB水平显著降低（p<0.05），而过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和超氧化物歧化酶的水平与仅给予酒精的组相比显著升高（p<0.05）。仅给予酒精的大鼠肝脏组织病理学检查显示，中央和中区出现严重坏死、单核细胞聚集和脂肪变性，这是肝脏受损的特征。用毛里求斯枣树叶水提取物或水飞蓟宾进行预处理可减少与长期酒精给药相关的形态学变化。植物提取物中观察到的单宁、皂苷和酚类化合物可能是观察到的降低损伤组织标志物水平和脂质过氧化作用的原因。
