Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt.
Nat Prod Res. 2022 Jan;36(1):136-141. doi: 10.1080/14786419.2020.1768089. Epub 2020 May 25.
The ethyl acetate fraction of the dried aerial parts of L. exhibited significant antimicrobial activity against some of selected bacteria and fungi. Also, it showed potent cytotoxicity against PANC-1 cancer cell lines under glucose deficient medium. The ethyl acetate fraction was subjected to different chromatographic techniques for isolation of the bioactive compounds. A new benzofuran glucoside; 2,3-dihydro-3-hydroxyeuparin 3-O-glucopyranoside () was isolated. Additionally, two known flavonoid compounds isorhamentin 3-O--D-glucoside (), and isorhamentin 3-O--D-rutinoside () were first identified in . Compound exhibited potent antimicrobial activities against two Gram-positive bacteria, one Gram-negative bacteria, and two fungi. Also, it displayed potent cytotoxic activity against PANC-1 cancer cell lines under glucose deficient medium (IC50 7.5 μM). However, the isolated flavonoid glycosides ( & ) showed moderate antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria, four fungi, and did not show any cytotoxic activity against PANC-1.
该研究从唇形科植物干燥地上部分的乙酸乙酯提取物中分离出一种新的苯并呋喃葡萄糖苷化合物 2,3-二氢-3-羟基野菰苷 3-O-吡喃葡萄糖苷 ()，该化合物对葡萄糖缺乏培养基中的 PANC-1 癌细胞系具有显著的细胞毒性（IC50 为 7.5 μM）。此外，从该植物中还首次分离得到两种已知的类黄酮化合物异荭草素 3-O--D-葡萄糖苷 () 和异荭草素 3-O--D-芦丁糖苷 ()。化合物 () 对两种革兰氏阳性菌、一种革兰氏阴性菌和两种真菌表现出较强的抗菌活性。同时，它在葡萄糖缺乏培养基中对 PANC-1 癌细胞系也表现出较强的细胞毒性（IC50 为 7.5 μM）。然而，分离得到的黄酮糖苷类化合物（ & ）对两种革兰氏阳性菌、两种革兰氏阴性菌、四种真菌表现出中等的抗菌活性，对 PANC-1 没有细胞毒性。
