University of Belgrade, Faculty of Chemistry, Studentski trg 12-16, 11000, Belgrade, Serbia.
University of Belgrade, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, Studentski trg 12-16, 11000, Belgrade, Serbia.
Phytochemistry. 2021 Jan;181:112580. doi: 10.1016/j.phytochem.2020.112580. Epub 2020 Nov 6.
Thirteen undescribed 24-methylene lanostane triterpenoids, named polyporenic acids E-M and fomitosides L-O, as well as seventeen known analogues, were isolated from the fruiting bodies of the mushroom Fomitopsis betulina. Their structures were determined using 1D, 2D NMR, IR, and HRESIMS. Fomitoside L and fomitoside N exhibited cytotoxicity against HL60 leukemia cells (IC = 15.8 and 23.7 μM, respectively). Among the known compounds, notable cytotoxicities against HL60 leukemia cells and selectivity with respect to MRC-5 healthy cells were noticed for dehydropachymic acid (IC = 10.9 μM, SI 8.6), pachymic acid (IC = 11.0 μM, SI 9.8), 3-epi-dehydrotumulosic acid (IC = 19.9 μM, SI 5.8) and 12α-hydroxy-3α-(3'-hydroxy-4'-methoxycarbonyl-3'-methylbutyryloxy)-24-methyllanosta-8,24 (31)-dien-26-oic acid (IC = 19.2 μM, SI 2.2).
从香菇 Fomitopsis betulina 的子实体中分离得到了 13 种未描述的 24-亚甲基羊毛甾烷三萜，分别命名为多孔酸 E-M 和 fomitosides L-O，以及 17 种已知类似物。它们的结构通过 1D、2D NMR、IR 和 HRESIMS 确定。Fomitoside L 和 fomitoside N 对 HL60 白血病细胞具有细胞毒性（IC = 15.8 和 23.7 μM）。在已知化合物中，脱水巴曲酸（IC = 10.9 μM，SI 8.6）、巴曲酸（IC = 11.0 μM，SI 9.8）、3-epi-脱氢松脂酸（IC = 19.9 μM，SI 5.8）和 12α-羟基-3α-（3'-羟基-4'-甲氧基羰基-3'-甲基丁酰氧基）-24-甲基羊毛甾烷-8,24（31）-二烯-26-酸（IC = 19.2 μM，SI 2.2）对 HL60 白血病细胞具有显著的细胞毒性和选择性。
