Zhou Lin, Chen He-Ping, Li Xinyang, Liu Ji-Kai
School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Pharmaceuticals (Basel). 2022 Dec 6;15(12):1520. doi: 10.3390/ph15121520.
Two triterpenes, ganoaustralins A () and B (), featuring unprecedented 6/6/6/5/6 scaffolds were isolated from the fruiting bodies of the mushroom . The structures were determined by extensive NMR and HRESIMS spectroscopic analysis. The absolute configuration of the C-25 in ganoaustralin A was assigned by the phenylglycine methyl ester (PGME) method. The relative and absolute configurations of the polycyclic backbones were determined by NMR and ECD calculations, respectively. The plausible biosynthetic pathways of ganoaustralins A and B were proposed. Ganoaustralin B showed weak inhibition against -secretase 1.
从该蘑菇子实体中分离出两种具有前所未有的6/6/6/5/6骨架的三萜类化合物，即灵芝澳大菌素A（）和B（）。通过广泛的核磁共振（NMR）和高分辨电喷雾电离质谱（HRESIMS）光谱分析确定了其结构。采用苯甘氨酸甲酯（PGME）法确定了灵芝澳大菌素A中C-25的绝对构型。通过核磁共振和电子圆二色（ECD）计算分别确定了多环骨架的相对构型和绝对构型。提出了灵芝澳大菌素A和B可能的生物合成途径。灵芝澳大菌素B对β-分泌酶1表现出微弱的抑制作用。
