Nguyen Ngan N T, McCarthy Colleen, Lantigua Darlin, Camci-Unal Gulden
Department of Chemical Engineering, University of Massachusetts Lowell, One University Avenue, Lowell, MA 01854, USA.
Biomedical Engineering and Biotechnology Program, University of Massachusetts Lowell, One University Avenue, Lowell, MA 01854, USA.
Diagnostics (Basel). 2020 Nov 5;10(11):905. doi: 10.3390/diagnostics10110905.
One of the most effective ways to prevent the spread of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is to develop accurate and rapid diagnostic tests. There are a number of molecular, serological, and imaging methods that are used to diagnose this infection in hospitals and clinical settings. The purpose of this review paper is to present the available approaches for detecting SARS-CoV-2 and address the advantages and limitations of each detection method. This work includes studies from recent literature publications along with information from the manufacturer's manuals of commercially available SARS-CoV-2 diagnostic products. Furthermore, supplementary information from the Food & Drug Administration (FDA), Centers for Disease Control and Prevention (CDC), and World Health Organization (WHO) is cited. The viral components targeted for virus detection, the principles of each diagnostic technique, and the detection efficiency of each approach are discussed. The potential of using diagnostic tests that were originally developed for previous epidemic viruses is also presented.
预防严重急性呼吸综合征冠状病毒2（SARS-CoV-2）传播的最有效方法之一是开发准确、快速的诊断测试。医院和临床环境中使用了多种分子、血清学和成像方法来诊断这种感染。这篇综述文章的目的是介绍检测SARS-CoV-2的现有方法，并阐述每种检测方法的优缺点。这项工作包括近期文献出版物中的研究以及市售SARS-CoV-2诊断产品制造商手册中的信息。此外，还引用了美国食品药品监督管理局（FDA）、疾病控制与预防中心（CDC）和世界卫生组织（WHO）的补充信息。文中讨论了用于病毒检测的病毒成分、每种诊断技术的原理以及每种方法的检测效率。还介绍了使用最初为先前流行病毒开发的诊断测试的潜力。
