B Santos Rita, Nascimento Rui, V Coelho Ana, Figueiredo Andreia
Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Plants (Basel). 2020 Nov 5;9(11):1498. doi: 10.3390/plants9111498.
Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. However, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by . Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, "Regent", in the first hours post inoculation (hpi) with . At 6 hpi, proteins related to defense and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of indicators of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defense processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defense strategy towards and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies for breeding approaches.
葡萄是世界上最重要的作物之一，可用于生产葡萄酒等具有重要经济价值的产品。然而，重要的葡萄品种受到霜霉病等病害的严重影响。葡萄抗性的提高主要通过育种技术实现，即将抗性性状导入具有所需葡萄特性的品种中。然而，对于抗性或耐性品种如何应对病原菌，我们仍然缺乏了解。在本研究中，我们采用鸟枪法蛋白质组学液相色谱-串联质谱（LC-MS/MS）方法，揭示了高耐性葡萄品种“瑞根特”在接种病原菌后的最初几个小时内的蛋白质调控情况。在接种后6小时，与防御和刺激反应相关的蛋白质受到负调控，而在接种后12小时，这两类蛋白质都有积累。在两个时间点都检测到效应子触发的易感性（ETS）和效应子触发的免疫（ETI）指标同时出现，表明这些防御过程具有高度可塑性。本研究获得的结果揭示了耐性葡萄对病原菌的防御策略，并可能为抗性相关候选基因和机制提供有价值的见解，这可能在制定新的育种策略中发挥重要作用。
