Department of Gynaecology and Obstetrics, The First People's Hospital of Lanzhou City, No. 1, Wujiayuan West Street. Qilihe District, Lanzhou, Gansu, 730050, China.
Department of Gynecology, Binzhou Chinese Medicine Hospital, Bincheng District, No. 539, Bohai 8th Road, Binzhou, Shandong, 256600, China.
Mol Cell Biochem. 2021 Feb;476(2):1051-1061. doi: 10.1007/s11010-020-03971-9. Epub 2020 Nov 10.
Long non-coding RNAs (lncRNAs) are known as crucial regulators in the development of OC. In the current study, we aim to explore the function and molecular mechanism of lncRNA DLEU1 in OC. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to determine the expression of DLEU1, miR-429, and TFAP2A in OC cells and tissues. The relationship among DLEU1, miR-429, and TFAP2A was tested by dual-luciferase reporter (DLR) assay. Besides, the proliferative, migratory and invasive abilities of OC cells were analyzed by MTT, wound healing, and transwell assays, respectively. Western blot was performed to determine the protein expression of TFAP2A. The expression of lncRNA DLEU1 and TFAP2A were upregulated, and miR-429 was downregulated in OC tissues. Silencing of DLEU1 inhibited the proliferation, migration, and invasion of OC cells. Bioinformation and DLR assay showed that DLEU1 acted as the sponge for miR-429. Moreover, miR-429 could directly target TFAP2A and inhibit the proliferation, migration, and invasion of OC cells. Moreover, we observed a negative correlation between miR-429 and DLEU1, and between miR-429 and TFAP2A in OC tissues. The transfection of miR-429 inhibitor or pcDNA-TFAP2A reversed the inhibitory effects of si-DLEU1 on the proliferation, migration, and invasion of OC cells. Silencing of DLEU1 inhibited the proliferation, migration, and invasion of OC cells by regulating miR-429/TFAP2A axis, indicating a potential therapeutic target for OC.
长链非编码 RNA（lncRNA）被认为是 OC 发生发展过程中的关键调控因子。在本研究中，我们旨在探讨 lncRNA DLEU1 在 OC 中的功能和分子机制。采用实时定量聚合酶链反应（qRT-PCR）检测 OC 细胞和组织中 DLEU1、miR-429 和 TFAP2A 的表达。通过双荧光素酶报告（DLR）实验验证 DLEU1、miR-429 和 TFAP2A 之间的关系。此外，通过 MTT、划痕愈合和 Transwell 实验分别分析 OC 细胞的增殖、迁移和侵袭能力。采用 Western blot 检测 TFAP2A 蛋白表达。OC 组织中 lncRNA DLEU1 和 TFAP2A 的表达上调，miR-429 表达下调。沉默 DLEU1 抑制 OC 细胞的增殖、迁移和侵袭。生物信息学和 DLR 实验表明，DLEU1 作为 miR-429 的海绵。此外，miR-429 可直接靶向 TFAP2A，抑制 OC 细胞的增殖、迁移和侵袭。此外，我们观察到 OC 组织中 miR-429 与 DLEU1 呈负相关，miR-429 与 TFAP2A 也呈负相关。转染 miR-429 抑制剂或 pcDNA-TFAP2A 逆转了 si-DLEU1 对 OC 细胞增殖、迁移和侵袭的抑制作用。沉默 DLEU1 通过调节 miR-429/TFAP2A 轴抑制 OC 细胞的增殖、迁移和侵袭，提示其可能成为 OC 的潜在治疗靶点。
