Department of Botany, PMAS Arid Agriculture University, Rawalpindi, Pakistan.
ZMBP-Department of Plant Biochemistry, University of Tuebingen, Tuebingen, Germany.
PLoS One. 2020 Nov 10;15(11):e0241568. doi: 10.1371/journal.pone.0241568. eCollection 2020.
The use of nanomaterials in agriculture is a current need and could be helpful in overcoming food security risks. Brassica napus L. is the third most important crop for edible oil, having double low unsaturated fatty acids. In the present study, we investigated the effects of green synthesized Zn NPs on biochemical effects, antioxidant enzymes, nutritional quality parameters and on the fatty acid profile of rapeseed (B. napus). Plant-mediated synthesis of zinc nanoparticles (Zn NPs) was carried out using Mentha arvensis L. leaf extract followed by characterization through ultraviolet-visible spectroscopy (UV-vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-Ray (EDX), and X-Ray diffraction (XRD). NPs exhibited irregular shapes ranging in size from 30-70 nm and EDX analysis confirmed 96.08% of Zn in the sample. The investigated biochemical characterization (protein content, proline content, total soluble sugar (TSS), total flavonoid content (TFC), and total phenolic content (TPC) showed a substantial change on exposure to Zn NPs. A dose-dependent gradual increase was observed in the antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Oil and moisture contents dropped significantly from the control level in the rapeseed (B. napus) varieties. However, different trends in nutritional (Zn, Na+, K+) and fatty acid profiling of B. napus have been noted. This study demonstrates that Zn NPs have the potential to improve the biochemical, nutritional, antioxidant enzymes, and fatty acid profile of B. napus varieties.
在农业中使用纳米材料是当前的需求，有助于克服食品安全风险。油菜（ Brassica napus L. ）是第三大重要的食用油作物，具有双低不饱和脂肪酸。在本研究中，我们研究了绿色合成的 Zn NPs 对油菜（ Brassica napus ）生化效应、抗氧化酶、营养品质参数和脂肪酸组成的影响。通过薄荷（ Mentha arvensis L. ）叶提取物进行植物介导的锌纳米粒子（ Zn NPs ）合成，随后通过紫外-可见光谱（ UV-vis ）、扫描电子显微镜（ SEM ）、透射电子显微镜（ TEM ）、能量色散 X 射线（ EDX ）和 X 射线衍射（ XRD ）进行表征。 NPs 呈现出 30-70nm 大小的不规则形状，EDX 分析证实样品中含有 96.08%的 Zn 。研究的生化特性（蛋白质含量、脯氨酸含量、总可溶性糖（ TSS ）、总类黄酮含量（ TFC ）和总酚含量（ TPC ）在暴露于 Zn NPs 时发生了显著变化。抗氧化酶超氧化物歧化酶（ SOD ）、过氧化物酶（ POD ）和过氧化氢酶（ CAT ）的剂量依赖性逐渐增加。油菜（ Brassica napus ）品种中的油分和水分含量与对照相比显著下降。然而，在油菜（ Brassica napus ）的营养（ Zn 、 Na+ 、 K+ ）和脂肪酸分布方面观察到了不同的趋势。本研究表明， Zn NPs 有可能改善油菜（ Brassica napus ）品种的生化特性、营养品质、抗氧化酶和脂肪酸组成。
