壳寡糖抑制核因子-κB 的激活，改善实验性自身免疫性葡萄膜炎小鼠的病情。
Chitosan Oligosaccharides Suppress Nuclear Factor-Kappa B Activation and Ameliorate Experimental Autoimmune Uveoretinitis in Mice.
机构信息
Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan.
Department of Ophthalmology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
出版信息
Int J Mol Sci. 2020 Nov 6;21(21):8326. doi: 10.3390/ijms21218326.
We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of COS or phosphate-buffered saline (PBS) was given to mice daily after EAU induction. We found that mouse EAU is ameliorated by the high-dose COS treatment when compared with PBS treatment. In the retinas of high-dose COS-treated mice, the nuclear translocation of NF-κB subunit (p65) was suppressed, and the expression of several key EAU inflammatory mediators, IFN-γ, TNF-α, IL-1α, IL-4, IL-5, IL-6, IL-10, IL-17 and MCP-1 was lowered. These results suggest that COS may be a potential treatment for posterior uveitis.
我们研究了壳寡糖（COS）在实验性自身免疫性葡萄膜炎（EAU）小鼠模型中的治疗潜力及其机制。通过向 C57/BL6 小鼠注射人眼间视网膜结合蛋白（IRBP）肽来诱导 EAU。同时，在 EAU 诱导后，每天给予 COS 高或低剂量（20 或 10mg/kg）或磷酸盐缓冲盐水（PBS）。我们发现，与 PBS 处理相比，COS 高剂量处理可改善小鼠 EAU。在高剂量 COS 处理的小鼠视网膜中，核易位的 NF-κB 亚基（p65）受到抑制，几种关键的 EAU 炎症介质 IFN-γ、TNF-α、IL-1α、IL-4、IL-5、IL-6、IL-10、IL-17 和 MCP-1 的表达水平降低。这些结果表明，COS 可能是治疗后葡萄膜炎的一种潜在方法。
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