Deutch A Y, Holliday J, Roth R H, Chun L L, Hawrot E
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8697-701. doi: 10.1073/pnas.84.23.8697.
A monoclonal antibody generated against purified acetylcholine receptor from Torpedo electric organ was used to immunohistochemically localize a neuronal nicotinic acetylcholine receptor. Regions of the rat brain stained with this antibody paralleled those areas of the brain exhibiting [3H]nicotine binding sites and corresponded to areas in which mRNAs encoding for alpha subunits of the neuronal nicotinic acetylcholine receptor are present. Thus, the anteroventral thalamus, cortex, hippocampus, medial habenula, interpeduncular nucleus, and substantia nigra/ventral tegmental area exhibited significant immunoreactivity. Neurons of the medial habenula and substantia nigra were densely stained, and processes were prominently delineated. Furthermore, in the projection areas of the medial habenula (interpeduncular nucleus and median raphe) axons were strongly immunoreactive and were distributed to distinct subdivisions of the target sites. The present data suggest that there are several discrete neuronal systems in which nicotinic acetylcholine receptors have functional importance. These immunohistochemical studies delineate at the single-cell level the localization within the mammalian central nervous system of certain nicotinic acetylcholine receptors.
一种针对从电鱼电器官中纯化的乙酰胆碱受体产生的单克隆抗体，被用于免疫组织化学定位神经元烟碱型乙酰胆碱受体。用该抗体染色的大鼠脑区与脑内显示[3H]尼古丁结合位点的区域平行，且对应于存在编码神经元烟碱型乙酰胆碱受体α亚基的mRNA的区域。因此，前腹侧丘脑、皮质、海马、内侧缰核、脚间核以及黑质/腹侧被盖区呈现出显著的免疫反应性。内侧缰核和黑质的神经元被密集染色，其突起清晰可辨。此外，在内侧缰核的投射区域（脚间核和中缝正中核），轴突具有强烈的免疫反应性，并分布于靶位点的不同亚区。目前的数据表明，存在几个离散的神经元系统，其中烟碱型乙酰胆碱受体具有功能重要性。这些免疫组织化学研究在单细胞水平上描绘了某些烟碱型乙酰胆碱受体在哺乳动物中枢神经系统中的定位。
