Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, China.
Reprod Domest Anim. 2021 Jan;56(1):161-171. doi: 10.1111/rda.13861. Epub 2020 Nov 23.
Seminal plasma is a complex biological fluid containing many metabolites including amino acids, fructose, carbohydrates and lipids Metabolites play important roles in multiple biological processes, but details and significance of the seminal plasma metabolome related to boar fertility are unknown. The aim of the present study was to compare the comprehensive metabolome of seminal plasma from boars with different conception rate after artificial insemination and to identify the potential biomarkers. Semen samples were collected from boars which divided into two groups according to the conception rates in the offspring. Seminal plasma metabolites were isolated, purified, and then subjected to Ultra-high Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC-qTOF-MS) procession. A total of 576 (Positive ion mode) and 377 (Negative ion mode) metabolites were identified in seminal plasma. Metabolites were identified and categorized according to their major chemical classes, including carboxylic acids and derivatives, organooxygen compounds, amino acids, peptides, and alogues, fatty amides, fatty acyls, benzene and substituted derivatives, purine nucleotides, pyrimidine nucleotides, glycosyl compounds, fatty acids and conjugates. The results showed that 4-Aminobenzoate, Pro-Asn, Ile-Tyr, Homoveratric acid and D-Biotin were higher in semen of boar with higher conception rate (HG) versus lower conception rate (LG) (p < .05), whereas L-Serine, Butoxyacetic acid, S-Methyl-5'-thioadenosine, Capsaicin and 1-O-(cis-9-Octadecenyl)-2-O-acetyl-sn-glycero-3-phosphocholine (PAF) were lower in HG than in LG (p < .05). These metabolites may be considered as candidate biomarkers for different fertility in boars.
精浆是一种复杂的生物流体，包含许多代谢物，包括氨基酸、果糖、碳水化合物和脂质。代谢物在多种生物过程中发挥重要作用，但与公猪生育力相关的精浆代谢组的细节和意义尚不清楚。本研究旨在比较人工授精后受胎率不同的公猪精浆的综合代谢组，并鉴定潜在的生物标志物。从根据后代受胎率分为两组的公猪中收集精液样本。分离、纯化精浆代谢物，然后进行超高效液相色谱-四极杆飞行时间质谱（UHPLC-qTOF-MS）处理。在精浆中鉴定出 576 种（正离子模式）和 377 种（负离子模式）代谢物。根据其主要化学类别对代谢物进行鉴定和分类，包括羧酸及其衍生物、含氧化合物、氨基酸、肽和类似物、脂肪酸酰胺、脂肪酸酰基、苯及其取代衍生物、嘌呤核苷酸、嘧啶核苷酸、糖基化合物、脂肪酸及其共轭物。结果表明，4-氨基苯甲酸、脯氨酸-天冬酰胺、异亮氨酸-酪氨酸、高香草酸和 D-生物素在受胎率较高（HG）的公猪精液中高于受胎率较低（LG）的公猪（p<0.05），而 L-丝氨酸、丁氧基乙酸、S-甲基-5'-硫代腺苷、辣椒素和 1-O-（顺-9-十八烯基）-2-O-乙酰-sn-甘油-3-磷酸胆碱（PAF）在 HG 中低于 LG（p<0.05）。这些代谢物可以被认为是公猪不同生育力的候选生物标志物。
