Zhang Shengdan, Niu Yulong, Yang Zhuo, Zhang Yuwei, Guo Qiang, Yang Yi, Zhou Xuedong, Ding Yi, Liu Chengcheng
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Exp Ther Med. 2020 Dec;20(6):251. doi: 10.3892/etm.2020.9381. Epub 2020 Oct 23.
Biochanin A (BA) is an organic compound produced by and with anti-inflammatory and antioxidative effects. The aim of the current study was to evaluate the effects of BA on gingival inflammation and alveolar bone destruction in rats with experimental periodontitis. Experimental rats (n=25) were distributed equally into five groups: i) Healthy control (control) group; ii) experimental periodontitis (ligation) group; and iii) and ligation plus low, medium and high dose of BA (12.5, 25 and 50 mg/kg/day, respectively) groups. A nylon ligature was inserted around rats' maxillary molars for 14 days to trigger the experimental periodontitis. BA was intravenous injected once daily for 4 weeks. After that, interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), reactive oxygen species (ROS) and osteocalcin (OCN) levels were determined in gingival and/or serum samples using ELISA or reverse transcription-quantitative PCR. Alveolar bone volume was assessed via hematoxylin and eosin staining and micro-computed tomography. Osteoclasts were identified by tartrate-resistant acid phosphatase staining, and the level of the nuclear factor erythroid-2 related factor 2 (Nrf2) was also detected by immunohistochemical staining. BA treatment groups showed alleviated alveolar bone resorption compared with the ligation group. Moreover, BA treatment significantly inhibited IL-1β, TNF-α, ROS levels, and reduced leukocyte acid phosphatase-positive cells, as well as increased OCN and Nrf2 levels compared with the ligation group. BA had beneficial effects on experimental periodontitis of rats. BA treatment inhibited inflammation, regulated unbalanced oxidative stress response and ameliorated the alveolar bone loss.
染料木素（BA）是一种由[具体产生物未给出]产生的有机化合物，具有抗炎和抗氧化作用。本研究的目的是评估BA对实验性牙周炎大鼠牙龈炎症和牙槽骨破坏的影响。将实验大鼠（n = 25）平均分为五组：i）健康对照组；ii）实验性牙周炎（结扎）组；iii）结扎加低、中、高剂量BA（分别为12.5、25和50 mg/kg/天）组。在大鼠上颌磨牙周围插入尼龙结扎线14天以引发实验性牙周炎。每天静脉注射BA一次，持续4周。之后，使用酶联免疫吸附测定（ELISA）或逆转录定量聚合酶链反应（RT-qPCR）测定牙龈和/或血清样本中的白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、活性氧（ROS）和骨钙素（OCN）水平。通过苏木精和伊红染色及显微计算机断层扫描评估牙槽骨体积。通过抗酒石酸酸性磷酸酶染色鉴定破骨细胞，并通过免疫组织化学染色检测核因子红细胞2相关因子2（Nrf2）水平。与结扎组相比，BA治疗组的牙槽骨吸收减轻。此外，与结扎组相比，BA治疗显著抑制IL-1β、TNF-α、ROS水平，减少白细胞酸性磷酸酶阳性细胞，并增加OCN和Nrf2水平。BA对大鼠实验性牙周炎具有有益作用。BA治疗可抑制炎症，调节失衡的氧化应激反应并改善牙槽骨丢失。
