Barber C, Eylan E
Zentralbl Bakteriol Orig A. 1976 Jan;234(1):46-52.
Groups of mice were immunized with detoxified protein from S. typhimurium, S. paratyphi B and S. paratyphi C. Consecutive infections with different concentrations of the homologous and heterologous strains showed that: 1. Immunizations with proteins from S. typhimurium induced protections in 65% of the mice infected with 50 LD100 of their natural pathogen, and in 80% of the mice infected with 50 LD100 of S. paratyphi B; the infection with S. paratyphi C of mice in this group afforded protection against 20 LD100 in 75% of the animals. 2. Immunization with proteins from S. paratyphi B induced protection in the mice against the infection with 20 LD100 of S. typhimurium (survival of 80% of the mice) and against 20 LD100 of the homologous S. paratyphi B (survival of 90% of the mice). 3. Immunization with proteins from S. paratyphi C protected the mice against the infection with 20 LD100 of S. typhimurium in a proportion of 80-85% of the animals; infection with the homologous S. paratyphi C did not result in protection against more than 20 LD100 of the bacteria (80-85% survivals). The survivors, in each group, when reinfected 30 days later with 50 LD100 of S. typhimurium resisted in a proportion of 100%, as a consequence of antibodies induced against more specific proteins released in the mice during the infections by the related pathogens.
将小鼠分成几组，用鼠伤寒沙门氏菌、副伤寒乙杆菌和副伤寒丙杆菌的解毒蛋白进行免疫。用不同浓度的同源和异源菌株进行连续感染，结果表明：1. 用鼠伤寒沙门氏菌的蛋白进行免疫，可使65%感染50个半数致死剂量（LD100）天然病原体的小鼠得到保护，80%感染50个LD100副伤寒乙杆菌的小鼠得到保护；该组小鼠感染副伤寒丙杆菌后，75%的动物对20个LD100有抵抗力。2. 用副伤寒乙杆菌的蛋白进行免疫，可使小鼠对20个LD100鼠伤寒沙门氏菌的感染产生抵抗力（80%的小鼠存活），对20个LD100同源副伤寒乙杆菌的感染产生抵抗力（90%的小鼠存活）。3. 用副伤寒丙杆菌的蛋白进行免疫，可使80 - 85%的动物对20个LD100鼠伤寒沙门氏菌的感染产生抵抗力；感染同源副伤寒丙杆菌对超过20个LD100的细菌无保护作用（80 - 85%存活）。每组中的存活小鼠在30天后再次感染50个LD100鼠伤寒沙门氏菌时，由于在感染相关病原体期间小鼠体内诱导产生了针对更特异性蛋白质的抗体，100%的小鼠具有抵抗力。
