Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California (UABC), Universidad 14418, Parque Internacional Industrial Tijuana, 22390, Tijuana, BC, Mexico.
Department of Cell Biology, Genetics and Physiology, University of Malaga, 29071, Malaga, Spain.
Biometals. 2021 Feb;34(1):107-117. doi: 10.1007/s10534-020-00267-9. Epub 2020 Nov 12.
Octyltrimethylammonium tetrathiotungstate salt (ATT-C8) was synthesized and its ability to chelate copper was evaluated. The biological and toxic aspects were evaluated by in vitro and in vivo assays, using bovine aorta endothelial cells (BAEC) and zebrafish (Danio rerio) embryos. The obtained results suggest that ATT-C8 has better biocompatibility, showing a significantly lower lethal concentration 50 (LC) value in comparison to ammonium tetrathiotungstate (ATT). Zebrafish embryos assay results indicate that both tetrathiotungstate salts at the studied concentrations increase the hatching time. Even more, an in vivo assay showed that synthesized materials behave as copper antagonists and have the ability to inhibit its toxicological effects. Also, both materials were found to be active for the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The characterization of the materials was carried out using the following spectroscopic techniques: Ultraviolet-Visible (UV-Vis), Fourier Transform Infrared (FTIR) and proton nuclear magnetic resonance (H-NRM).
辛基三甲基铵四硫代钨酸盐盐（ATT-C8）被合成，并评估了其螯合铜的能力。通过体外和体内试验，使用牛主动脉内皮细胞（BAEC）和斑马鱼（Danio rerio）胚胎评估了其生物和毒性方面。获得的结果表明，ATT-C8 具有更好的生物相容性，与四硫代钨酸铵（ATT）相比，其半数致死浓度（LC）值明显更低。斑马鱼胚胎试验结果表明，在所研究浓度下，两种四硫代钨酸盐盐都增加了孵化时间。更重要的是，体内试验表明，合成材料表现为铜拮抗剂，具有抑制其毒性作用的能力。此外，这两种材料在体外 2,2-二苯基-1-苦基肼（DPPH）试验中均表现出活性。使用以下光谱技术对材料进行了表征：紫外-可见（UV-Vis）、傅里叶变换红外（FTIR）和质子核磁共振（H-NRM）。
