使用斑马鱼模型评估胚胎毒性。
Evaluation of embryotoxicity using the zebrafish model.
作者信息
Truong Lisa, Harper Stacey L, Tanguay Robert L
机构信息
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
出版信息
Methods Mol Biol. 2011;691:271-9. doi: 10.1007/978-1-60761-849-2_16.
Abstract
The embryonic zebrafish model offers the power of whole-animal investigations (e.g., intact organism, functional homeostatic feedback mechanisms, and intercellular signaling) with the convenience of cell culture (e.g., cost- and time-efficient, minimal infrastructure, small quantities of nanomaterial solutions required). The model system overcomes many of the current limitations in rapid to high-throughput screening of drugs/compounds and casts a broad net to evaluate integrated system effects rapidly. Additionally, it is an ideal platform to follow up with targeted studies aimed at the mechanisms of toxic action. Exposures are carried out in 96-well plates so minimal solution volumes are required for the assessments. Numerous morphological, developmental, and behavioral endpoints can be evaluated noninvasively due to the transparent nature of the embryos.
摘要
斑马鱼胚胎模型兼具全动物研究的优势（如完整的生物体、功能性稳态反馈机制和细胞间信号传导）以及细胞培养的便利性（如成本低、耗时短、所需基础设施少、所需纳米材料溶液量少）。该模型系统克服了当前在药物/化合物快速高通量筛选中的许多局限性，能够广泛地快速评估综合系统效应。此外，它是后续针对毒性作用机制进行靶向研究的理想平台。暴露实验在96孔板中进行，因此评估所需的溶液体积最小。由于胚胎具有透明的特性，许多形态学、发育和行为学终点都可以进行无创评估。
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