Rota P A, Shaw M W, Kendal A P
Division of Viral Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Virology. 1987 Dec;161(2):269-75. doi: 10.1016/0042-6822(87)90118-8.
To compare the immune response induced by influenza hemagglutinin (HA) variants differing by a single amino acid, the genes for each HA variant were cloned and expressed in vaccinia virus. These variant HA genes have been previously described as being present exclusively in either egg-derived or MDCK cell-derived subpopulations of influenza B/England/222/82 virus. By using this approach we were able to vaccinate animals with homogeneous preparations of these viral antigens and thus circumvent the problem of heterogeneity within RNA virus stocks. Immunization and challenge experiments in mice indicated that even though vaccination with the recombinant vaccinia viruses induced different levels of cross-reactive neutralizing antibodies, mice vaccinated with either recombinant vaccinia virus were protected from infection with either subpopulation of influenza virus. Results with this model system support the view that influenza vaccines prepared with egg-derived virus should be protective against microvariants of virus that grow preferentially in MDCK and possibly other mammalian cells.
为了比较仅相差一个氨基酸的流感血凝素（HA）变体所诱导的免疫反应，将每个HA变体的基因克隆并在痘苗病毒中表达。这些变体HA基因先前已被描述为仅存在于乙型流感病毒/英格兰/222/82的鸡胚来源或MDCK细胞来源的亚群中。通过使用这种方法，我们能够用这些病毒抗原的均质制剂对动物进行免疫接种，从而规避RNA病毒株内异质性的问题。小鼠的免疫和攻毒实验表明，尽管用重组痘苗病毒接种诱导了不同水平的交叉反应性中和抗体，但用任何一种重组痘苗病毒接种的小鼠都受到保护，免受任何一种流感病毒亚群的感染。该模型系统的结果支持这样一种观点，即用鸡胚来源病毒制备的流感疫苗应对优先在MDCK细胞及可能的其他哺乳动物细胞中生长的病毒微变体具有保护作用。
