Robertson J S, Bootman J S, Nicolson C, Major D, Robertson E W, Wood J M
National Institute for Biological Standards and Control, South Mimms, Herts, United Kingdom.
Virology. 1990 Nov;179(1):35-40. doi: 10.1016/0042-6822(90)90270-2.
When clinical specimens of influenza virus are adapted to grow in embryonated hens' eggs, variants are selected which have specific amino acid substitutions in the hemagglutinin (HA). In contrast, a single virus, distinct from any egg-adapted variant, is selected when virus is isolated on MDCK mammalian cell culture. We have utilized the polymerase chain reaction to determine the nature of the hemagglutinin of influenza B viruses present in clinical material prior to cultivation in the laboratory. Sequence analysis of individual clones of amplified DNA reveals that the HA of clinical virus is essentially homogeneous and identical to the virus derived on MDCK cells. The HA of egg-adapted virus was heterogeneous and nonidentical to that of the clinical material and of the MDCK-derived virus.
当流感病毒的临床标本适应在鸡胚中生长时，会选择在血凝素（HA）中具有特定氨基酸取代的变体。相比之下，当病毒在MDCK哺乳动物细胞培养物上分离时，会选择一种不同于任何适应鸡蛋变体的单一病毒。我们利用聚合酶链反应来确定在实验室培养之前临床材料中存在的乙型流感病毒血凝素的性质。对扩增DNA的单个克隆进行序列分析表明，临床病毒的HA基本同质，且与在MDCK细胞上衍生的病毒相同。适应鸡蛋的病毒的HA是异质的，与临床材料和MDCK衍生病毒的HA不同。
