Department of Biomedical Engineering, New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan, 48513, Republic of Korea.
Department of Biomedical Engineering, New-senior Healthcare Innovation Center (BK21 Plus), Pukyong National University, Busan, 48513, Republic of Korea.
Carbohydr Polym. 2021 Jan 15;252:117145. doi: 10.1016/j.carbpol.2020.117145. Epub 2020 Sep 28.
In the present study, chitooligosaccharide (COS) and salicylic acid (SA) conjugates were synthesized using HO-induced grafting polymerization and oxidized alginate (OA) and gelatin hydrogels were fabricated using Schiff's base reaction. To confirm the synthesis and improved activity of the COS-SA conjugates, polyphenol assays, ultraviolet absorbance, NMR, and antioxidant activity tests were all conducted. The results showed that the COS-SA conjugates were completely synthesized and improved antioxidant activity. OA was synthesized using a periodate method and a hydrogel was obtained at a mixed ratio of 3:7 via crosslinking between the aldehyde group of the OA and the amine groups of gelatin with COS-SA conjugates. In a cytotoxicity and wound healing model, the hydrogels with COS-SA conjugates did not exhibit cytotoxicity and exhibited wound healing activity in a mouse model. These results provide evidence that OA and gelatin hydrogels with a COS-SA conjugate can be successfully used for wound healing applications.
在本研究中，采用 HO 诱导接枝聚合合成了壳寡糖（COS）和水杨酸（SA）缀合物，并通过席夫碱反应制备了氧化海藻酸钠（OA）和明胶水凝胶。为了确认 COS-SA 缀合物的合成和提高的活性，进行了多酚测定、紫外吸收、NMR 和抗氧化活性测试。结果表明，COS-SA 缀合物完全合成并提高了抗氧化活性。OA 是通过高碘酸盐法合成的，通过 OA 的醛基与含有 COS-SA 缀合物的明胶的氨基之间的交联，在 3:7 的混合比下获得水凝胶。在细胞毒性和伤口愈合模型中，含有 COS-SA 缀合物的水凝胶没有表现出细胞毒性，并在小鼠模型中表现出伤口愈合活性。这些结果表明，含有 COS-SA 缀合物的 OA 和明胶水凝胶可成功用于伤口愈合应用。
