Poison Control and Forensic Chemistry Center, Ministry of Health, P.O. Box 42351, Medina, Kingdom of Saudi Arabia.
Department of Exercise Physiology, College of Sport Sciences and Physical Activity, King Saud University, Building 69, P.O. Box 1949, 11441, Riyadh, Kingdom of Saudi Arabia.
Sci Rep. 2020 Nov 12;10(1):19759. doi: 10.1038/s41598-020-75755-6.
Metabolomics profiling was carried out to observe the effect of short-term intensive physical activity on the metabolome of young Saudi professional football players. Urine, plasma and saliva were collected on 2 days pre- and post-training. An Orbitrap Exactive mass spectrometer was used to analyze the samples. A reversed-phase (RP) column was used for the analysis of non-polar plasma metabolites, and a ZIC-pHILIC column was used for the analysis of plasma, saliva and urine. mzMine was used to extract the data, and the results were modelled using Simca-P 14.1 software. There was no marked variation in the metabolite profiles between pre day 1 and 2 or between post day 1 and 2 according to principal components analysis (PCA). When orthogonal partial least squares (OPLSDA) modelling was also used, and then models could be fitted based on a total number of metabolites of 75, 16 and 32 for urine, plasma and saliva using hydrophilic interaction chromatography (HILIC) and 6 for analysis of plasma with reversed-phase (RP) chromatography respectively. The present study concludes that acylcarnitine may increase post-exercise in football players suggesting that they may burn fat rather than glucose. The levels of carnitine metabolites in plasma post-exercise could provide an important indicator of fitness.
进行代谢组学分析，观察短期强化体育活动对沙特年轻职业足球运动员代谢组的影响。在训练前和训练后 2 天采集尿液、血浆和唾液样本。使用 Orbitrap Exactive 质谱仪分析样品。采用反相（RP）柱分析非极性血浆代谢物，采用 ZIC-pHILIC 柱分析血浆、唾液和尿液。使用 mzMine 提取数据，使用 Simca-P 14.1 软件对结果进行建模。根据主成分分析（PCA），第 1 天和第 2 天之间或第 1 天和第 2 天之间的代谢物谱没有明显变化。当使用正交偏最小二乘判别分析（OPLSDA）建模时，然后可以基于亲水相互作用色谱（HILIC）分别为尿液、血浆和唾液拟合总共 75、16 和 32 个代谢物和为分析血浆拟合 6 个代谢物的模型。本研究的结论是，酰基肉碱可能会在足球运动员运动后增加，表明他们可能燃烧脂肪而不是葡萄糖。运动后血浆肉碱代谢物的水平可以提供一个重要的健康指标。
