Bu Fanqin, Zhu Xiaojian, Yi Xuan, Luo Chen, Lin Kang, Zhu Jinfeng, Hu Cegui, Liu Zitao, Zhao Jiefeng, Huang Chao, Zhang Wenjun, Huang Jun
Department of Gastrointestinal Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330006, People's Republic of China.
Jiangxi Medical College of Nanchang University, Nanchang, People's Republic of China.
Onco Targets Ther. 2020 Nov 6;13:11433-11444. doi: 10.2147/OTT.S275649. eCollection 2020.
The GINS complex has been implicated in the prognosis of various cancers. It comprises four subunits, encoded by , , , and genes. Based on the current understanding, no report exists on the role of the GINS complex in pancreatic cancer.
We employed various bioinformatics databases including GEPIA, UALCAN, GEPIA2, and Kaplan Meier Plotter to identify the expression profile of the four genes (, , , and ), their correlation with pancreatic cancer grade as well as their prognostic value of in pancreatic cancer. Western blotting and qRT-PCR analyses were conducted to verify the expression profiles of the four genes in pancreatic cancer. CCK8 and EdU cell experiments were conducted to reveal the role played by the four genes in pancreatic cancer cell proliferation.
Based on GEPIA, Western blotting, and qRT-PCR analyses, all the four genes in the GINS complex were overexpressed in pancreatic cancer. Notably, the expression of each member was significantly associated with pancreatic cancer grade. The prognostic analysis revealed that not only the whole GINS complex but also each individual were prognostic biomarkers for pancreatic cancer. CCK8 and EdU experiments demonstrated that inhibition of the expression of each GINS member lowered pancreatic cancer cell proliferation.
This work implicated , , , and genes as critical prognostic markers for pancreatic cancer.
GINS复合物与多种癌症的预后有关。它由四个亚基组成，分别由、、和基因编码。基于目前的了解，尚无关于GINS复合物在胰腺癌中作用的报道。
我们使用了包括GEPIA、UALCAN、GEPIA2和Kaplan Meier Plotter在内的各种生物信息学数据库，以确定这四个基因（、、和）的表达谱、它们与胰腺癌分级的相关性以及它们在胰腺癌中的预后价值。进行了蛋白质免疫印迹和qRT-PCR分析，以验证这四个基因在胰腺癌中的表达谱。进行了CCK8和EdU细胞实验，以揭示这四个基因在胰腺癌细胞增殖中所起的作用。
基于GEPIA、蛋白质免疫印迹和qRT-PCR分析，GINS复合物中的所有四个基因在胰腺癌中均过表达。值得注意的是，每个成员的表达都与胰腺癌分级显著相关。预后分析表明，不仅整个GINS复合物，而且每个个体都是胰腺癌的预后生物标志物。CCK8和EdU实验表明，抑制每个GINS成员的表达会降低胰腺癌细胞的增殖。
这项研究表明、、和基因是胰腺癌的关键预后标志物。
