Leigh Noel J, Goniewicz Maciej L
Department of Health Behavior, Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States.
Front Physiol. 2020 Oct 21;11:592321. doi: 10.3389/fphys.2020.592321. eCollection 2020.
: Although electronic cigarettes (e-cigarettes) were originally developed to deliver aerosolized nicotine to lungs, recent data have shown that consumers also use them for inhalation of other drugs, including cannabidiol (CBD). The aim of this study was to test the acute inhalation toxicity of flavored CBD-containing aerosols emitted from e-cigarettes. : Bronchial epithelial cells (H292) cells were exposed to aerosol generated from e-cigarettes refilled either with (1) propylene glycol solvent only (PG, control), (2) commercially purchased unflavored solution with CBD, or (3) commercially purchased solutions with and without CBD and with different flavors. The toxicological effects were assessed using the following methods: (1) trypan blue exclusion assay (cell viability), (2) neutral red uptake assay (metabolic activity), and (3) ELISA (concentrations of inflammatory mediators). : Most flavored products with or without CBD were cytotoxic as compared to the air control. Overall, aerosols with CBD were more cytotoxic than aerosols without CBD irrelevant of the flavoring used in the product. Although, unflavored aerosols containing CBD in PG were significantly more cytotoxic than aerosols containing only PG, not all flavored products containing CBD were significantly more toxic than the same flavored products without CBD. Most CBD containing products significantly increase the concentration of cytokines released as compared to the same flavored products without CBD. : Different flavors show different cytotoxic effects in CBD-containing e-cigarettes. Aerosols emitted from CBD containing e-cigarettes were more cytotoxic than those emitted from CBD-free e-cigarettes.
尽管电子烟最初是为将雾化尼古丁输送到肺部而开发的，但最近的数据表明，消费者也使用它们来吸入其他药物，包括大麻二酚（CBD）。本研究的目的是测试电子烟释放的含调味CBD气雾剂的急性吸入毒性。支气管上皮细胞（H292）暴露于由以下电子烟重新填充产生的气雾剂中：（1）仅含丙二醇溶剂（PG，对照），（2）市售无调味CBD溶液，或（3）市售含或不含CBD且有不同口味的溶液。使用以下方法评估毒理学效应：（1）台盼蓝排斥试验（细胞活力），（2）中性红摄取试验（代谢活性），以及（3）酶联免疫吸附测定（炎症介质浓度）。与空气对照相比，大多数含或不含CBD的调味产品都具有细胞毒性。总体而言，含CBD的气雾剂比不含CBD的气雾剂细胞毒性更大，与产品中使用的调味剂无关。尽管PG中含CBD的无调味气雾剂比仅含PG的气雾剂细胞毒性显著更大，但并非所有含CBD的调味产品都比不含CBD的相同调味产品毒性显著更大。与不含CBD的相同调味产品相比，大多数含CBD的产品显著增加了释放的细胞因子浓度。不同口味在含CBD的电子烟中表现出不同的细胞毒性作用。含CBD的电子烟释放的气雾剂比不含CBD的电子烟释放的气雾剂细胞毒性更大。
